BD PharmingenTM APC Mouse Anti-Human Integrin alpha1 (CD49a)

Description

Multiparameter flow cytometric analysis of Integrin alpha1 CD49a expression on Human leucocyte populations. Human whole blood was stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 569036/569037; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the Human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; dashed line histogram or APC Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 569036/569037; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Integrin alpha1 CD49a expression on Human leucocyte populations. Human whole blood was stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 569036/569037; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the Human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; dashed line histogram or APC Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 569036/569037; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of Integrin alpha1 CD49a expression on Human leucocyte populations. Human whole blood was stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 569036/569037; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of Integrin alpha1 CD49a or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Integrin alpha1 CD49a expression on HeLa cells. Cells from the Human HeLa Adenocarcinoma, ATCC CCL-2 cell line were stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; dashed line histogram or APC Mouse Anti-Human Integrin alpha1 CD49a antibody Cat. No. 569036/569037; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Integrin alpha1 CD49a expression or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software.