BD PharmingenTM APC Mouse Anti-Human MIP-1alpha (CCL3)

Description

Flow cytometric analysis of MIP-1alpha CCL3 expression on stimulated Rhesus macaque Macaca mulatta peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN- 10 ng/ml, 2 hours , then LPS 1 mug/ml, 2 hours in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1alpha CCL3 Cat. No. 551533 . Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes. Flow cytometric analysis of MIP-1alpha CCL3 expression on stimulated Rhesus macaque Macaca mulatta peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN- 10 ng/ml, 2 hours , then LPS 1 mug/ml, 2 hours in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1alpha CCL3 Cat. No. 551533 . Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes. Show More Flow cytometric analysis of MIP-1alpha CCL3 expression on stimulated Rhesus macaque Macaca mulatta peripheral blood monocytes. Rhesus PBMC were stimulated with rhIFN- 10 ng/ml, 2 hours , then LPS 1 mug/ml, 2 hours in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were harvested, stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397 , fixed, permeabilized, and subsequently stained with APC Mouse Anti-Human MIP-1alpha CCL3 Cat. No. 551533 . Two-color dot plot was derived from gated events with the forward and side-scattering characteristics of viable monocytes.