BD PharmingenTM APC Mouse Anti-Human TCR alphabeta

Description

Multicolor flow cytometric analysis of TCR alphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV737 Mouse Anti-Human CD19 antibody Cat. No. 612756; Top Plots , BD Horizon BB515 Mouse Anti-Human CD3 antibody Cat. No. 564465; Bottom Plots and with either APC Mouse IgG1, kappa Isotype Control Cat No. 554681; Left Plots or APC Mouse Anti-Human TCR alphabeta antibody Cat. No. 571654/571733; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of TCR alphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCR alphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of TCR alphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV737 Mouse Anti-Human CD19 antibody Cat. No. 612756; Top Plots , BD Horizon BB515 Mouse Anti-Human CD3 antibody Cat. No. 564465; Bottom Plots and with either APC Mouse IgG1, kappa Isotype Control Cat No. 554681; Left Plots or APC Mouse Anti-Human TCR alphabeta antibody Cat. No. 571654/571733; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of TCR alphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCR alphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of TCR alphabeta expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV737 Mouse Anti-Human CD19 antibody Cat. No. 612756; Top Plots , BD Horizon BB515 Mouse Anti-Human CD3 antibody Cat. No. 564465; Bottom Plots and with either APC Mouse IgG1, kappa Isotype Control Cat No. 554681; Left Plots or APC Mouse Anti-Human TCR alphabeta antibody Cat. No. 571654/571733; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Top Plots: Bivariate pseudocolor density plots showing the correlated expression of TCR alphabeta or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Bottom Plots: Bivariate pseudocolor density plots showing the correlated expression of TCR alphabeta or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes.