BD PharmingenTM APC Mouse Anti-Human TNF

Description

Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were stained with FITC Mouse Anti-Human CD3 Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC Mouse Anti-Human TNF Cat. No. 554514; left panel . To demonstrate specificity of staining, the binding of APC Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with recombinant human TNF 0.5 mug; Cat. No. 554618; see middle panel , and by preincubation of the fixed/permeabilized cells with the Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; right panel prior to staining with the APC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine-blocking and unlabeled antibody-blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were stained with FITC Mouse Anti-Human CD3 Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC Mouse Anti-Human TNF Cat. No. 554514; left panel . To demonstrate specificity of staining, the binding of APC Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with recombinant human TNF 0.5 mug; Cat. No. 554618; see middle panel , and by preincubation of the fixed/permeabilized cells with the Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; right panel prior to staining with the APC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine-blocking and unlabeled antibody-blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were stained with FITC Mouse Anti-Human CD3 Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC Mouse Anti-Human TNF Cat. No. 554514; left panel . To demonstrate specificity of staining, the binding of APC Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with recombinant human TNF 0.5 mug; Cat. No. 554618; see middle panel , and by preincubation of the fixed/permeabilized cells with the Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; right panel prior to staining with the APC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine-blocking and unlabeled antibody-blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were stained with FITC Mouse Anti-Human CD3 Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC Mouse Anti-Human TNF Cat. No. 554514; left panel . To demonstrate specificity of staining, the binding of APC Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with recombinant human TNF 0.5 mug; Cat. No. 554618; see middle panel , and by preincubation of the fixed/permeabilized cells with the Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; right panel prior to staining with the APC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine-blocking and unlabeled antibody-blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were stained with FITC Mouse Anti-Human CD3 Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC Mouse Anti-Human TNF Cat. No. 554514; left panel . To demonstrate specificity of staining, the binding of APC Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with recombinant human TNF 0.5 mug; Cat. No. 554618; see middle panel , and by preincubation of the fixed/permeabilized cells with the Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; right panel prior to staining with the APC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine-blocking and unlabeled antibody-blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop Cat. No. 554724; aka monensin 2 muM . The PBMC were stained with FITC Mouse Anti-Human CD3 Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC Mouse Anti-Human TNF Cat. No. 554514; left panel . To demonstrate specificity of staining, the binding of APC Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with recombinant human TNF 0.5 mug; Cat. No. 554618; see middle panel , and by preincubation of the fixed/permeabilized cells with the Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; right panel prior to staining with the APC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine-blocking and unlabeled antibody-blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur.