BD PharmingenTM APC Mouse Anti-Mouse CD159a (NKG2AB6)

Description

Multicolor flow cytometric analysis of Mouse strain-specific expression of CD159a NKG2AB6 on splenic NK cells. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857/561067 and with either APC Mouse IgG2b, kappa Isotype Control Cat. No. 565381; Left Plots or APC Mouse Anti-Mouse CD159a NKG2AB6 antibody Cat. No. 569527/569528; Right Plots at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD159a NKG2AB6 or Ig Isotype control staining versus CD49b were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Mouse strain-specific expression of CD159a NKG2AB6 on splenic NK cells. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857/561067 and with either APC Mouse IgG2b, kappa Isotype Control Cat. No. 565381; Left Plots or APC Mouse Anti-Mouse CD159a NKG2AB6 antibody Cat. No. 569527/569528; Right Plots at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD159a NKG2AB6 or Ig Isotype control staining versus CD49b were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Mouse strain-specific expression of CD159a NKG2AB6 on splenic NK cells. C57BL/6 Top Plots and BALB/c Bottom Plots Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with FITC Rat Anti-Mouse CD49b antibody Cat. No. 553857/561067 and with either APC Mouse IgG2b, kappa Isotype Control Cat. No. 565381; Left Plots or APC Mouse Anti-Mouse CD159a NKG2AB6 antibody Cat. No. 569527/569528; Right Plots at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plots showing the correlated expression of CD159a NKG2AB6 or Ig Isotype control staining versus CD49b were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.