BD PharmingenTM APC Mouse Anti-Mouse CD64[a,b]

Description

Multicolor flow cytometric analysis of CD64 a,b expression on viable Mouse bone marrow cells. Mouse bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes and washed. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV496 Rat Anti-CD11b antibody Cat. No. 569708 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Mouse CD64 a,b antibody Cat. No. 571700/571772; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD64 a,b or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD64 a,b expression on viable Mouse bone marrow cells. Mouse bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes and washed. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV496 Rat Anti-CD11b antibody Cat. No. 569708 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Mouse CD64 a,b antibody Cat. No. 571700/571772; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD64 a,b or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD64 a,b expression on viable Mouse bone marrow cells. Mouse bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes and washed. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV496 Rat Anti-CD11b antibody Cat. No. 569708 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Mouse CD64 a,b antibody Cat. No. 571700/571772; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD64 a,b or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD64 a,b expression on viable Mouse bone marrow cells. Mouse bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes and washed. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV496 Rat Anti-CD11b antibody Cat. No. 569708 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Mouse CD64 a,b antibody Cat. No. 571700/571772; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD64 a,b or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD64 a,b expression on viable Mouse bone marrow cells. Mouse bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes and washed. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV496 Rat Anti-CD11b antibody Cat. No. 569708 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Mouse CD64 a,b antibody Cat. No. 571700/571772; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD64 a,b or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD64 a,b expression on viable Mouse bone marrow cells. Mouse bone marrow cells were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes and washed. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV496 Rat Anti-CD11b antibody Cat. No. 569708 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Mouse CD64 a,b antibody Cat. No. 571700/571772; Right Plot at 1 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD64 a,b or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.