BD PharmingenTM APC Mouse IgG1 kappa Isotype Control

Description

Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma and calcium ionophore A23187 1 mug/ml final concentration; Sigma , in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PMBC were stained with FITC-anti-CD3 FITC-UCHT1, Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.125 mug of APC-mouse anti-human TNF APC-MAb11, Cat. No. 554514, left panel or with 0.125 mug APC-mouse IgG1 Cat. No. 554681, right panel using BD Pharmingen staining protocol. To demonstrate specificity, the binding of APC-MAb11 antibody was blocked by perincubation of fixed/permabilized cells with excess unlabelled MAb11 antibody 5 mug; Cat. No. 554510 . The quadrant markers for the bivariate dot plot were set based on isotype controls and verified using the unlabelled MAb11 antibody blocking control. The APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACSVantage, or FACSCalibur. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma and calcium ionophore A23187 1 mug/ml final concentration; Sigma , in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PMBC were stained with FITC-anti-CD3 FITC-UCHT1, Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.125 mug of APC-mouse anti-human TNF APC-MAb11, Cat. No. 554514, left panel or with 0.125 mug APC-mouse IgG1 Cat. No. 554681, right panel using BD Pharmingen staining protocol. To demonstrate specificity, the binding of APC-MAb11 antibody was blocked by perincubation of fixed/permabilized cells with excess unlabelled MAb11 antibody 5 mug; Cat. No. 554510 . The quadrant markers for the bivariate dot plot were set based on isotype controls and verified using the unlabelled MAb11 antibody blocking control. The APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACSVantage, or FACSCalibur. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma and calcium ionophore A23187 1 mug/ml final concentration; Sigma , in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PMBC were stained with FITC-anti-CD3 FITC-UCHT1, Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.125 mug of APC-mouse anti-human TNF APC-MAb11, Cat. No. 554514, left panel or with 0.125 mug APC-mouse IgG1 Cat. No. 554681, right panel using BD Pharmingen staining protocol. To demonstrate specificity, the binding of APC-MAb11 antibody was blocked by perincubation of fixed/permabilized cells with excess unlabelled MAb11 antibody 5 mug; Cat. No. 554510 . The quadrant markers for the bivariate dot plot were set based on isotype controls and verified using the unlabelled MAb11 antibody blocking control. The APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACSVantage, or FACSCalibur. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma and calcium ionophore A23187 1 mug/ml final concentration; Sigma , in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PMBC were stained with FITC-anti-CD3 FITC-UCHT1, Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.125 mug of APC-mouse anti-human TNF APC-MAb11, Cat. No. 554514, left panel or with 0.125 mug APC-mouse IgG1 Cat. No. 554681, right panel using BD Pharmingen staining protocol. To demonstrate specificity, the binding of APC-MAb11 antibody was blocked by perincubation of fixed/permabilized cells with excess unlabelled MAb11 antibody 5 mug; Cat. No. 554510 . The quadrant markers for the bivariate dot plot were set based on isotype controls and verified using the unlabelled MAb11 antibody blocking control. The APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACSVantage, or FACSCalibur. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma and calcium ionophore A23187 1 mug/ml final concentration; Sigma , in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PMBC were stained with FITC-anti-CD3 FITC-UCHT1, Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.125 mug of APC-mouse anti-human TNF APC-MAb11, Cat. No. 554514, left panel or with 0.125 mug APC-mouse IgG1 Cat. No. 554681, right panel using BD Pharmingen staining protocol. To demonstrate specificity, the binding of APC-MAb11 antibody was blocked by perincubation of fixed/permabilized cells with excess unlabelled MAb11 antibody 5 mug; Cat. No. 554510 . The quadrant markers for the bivariate dot plot were set based on isotype controls and verified using the unlabelled MAb11 antibody blocking control. The APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACSVantage, or FACSCalibur. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA 50 ng/ml final concentration; Sigma and calcium ionophore A23187 1 mug/ml final concentration; Sigma , in the presence of GolgiStop 2 muM final concentration; Cat. No. 554724 . The PMBC were stained with FITC-anti-CD3 FITC-UCHT1, Cat. No. 555332 , fixed, permeabilized, and subsequently stained with 0.125 mug of APC-mouse anti-human TNF APC-MAb11, Cat. No. 554514, left panel or with 0.125 mug APC-mouse IgG1 Cat. No. 554681, right panel using BD Pharmingen staining protocol. To demonstrate specificity, the binding of APC-MAb11 antibody was blocked by perincubation of fixed/permabilized cells with excess unlabelled MAb11 antibody 5 mug; Cat. No. 554510 . The quadrant markers for the bivariate dot plot were set based on isotype controls and verified using the unlabelled MAb11 antibody blocking control. The APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACSVantage, or FACSCalibur.