BD PharmingenTM APC Rat Anti-Human IL-6

Description

Flow cytometric analysis of IL-6 expression by stimulated human monocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with lipopolysaccharide LPS; 100 ng/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor Containing Monensin 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with PE-Mouse Anti-Human CD14 monoclonal antibody Cat. No. 555398 , fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 , and stained with APC Rat Anti-Human IL-6 antibody Cat. No. 561441, solid line histogram or an APC Rat IgG1, kappa Isotype Control Cat No. 554686, dashed line histogram . The fluorescence histograms were derived from CD14-positive events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IL-6 expression by stimulated human monocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with lipopolysaccharide LPS; 100 ng/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor Containing Monensin 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with PE-Mouse Anti-Human CD14 monoclonal antibody Cat. No. 555398 , fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 , and stained with APC Rat Anti-Human IL-6 antibody Cat. No. 561441, solid line histogram or an APC Rat IgG1, kappa Isotype Control Cat No. 554686, dashed line histogram . The fluorescence histograms were derived from CD14-positive events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IL-6 expression by stimulated human monocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with lipopolysaccharide LPS; 100 ng/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor Containing Monensin 2 muM final concentration; Cat. No. 554724 . The PBMC were harvested, stained with PE-Mouse Anti-Human CD14 monoclonal antibody Cat. No. 555398 , fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Perm/Wash Buffer Cat. No. 554723 , and stained with APC Rat Anti-Human IL-6 antibody Cat. No. 561441, solid line histogram or an APC Rat IgG1, kappa Isotype Control Cat No. 554686, dashed line histogram . The fluorescence histograms were derived from CD14-positive events with the forward and side light-scatter characteristics of intact monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.