BD PharmingenTM APC Rat Anti-Mouse CD127

Description

Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Panel or APC Rat Anti-Mouse CD127 antibody Cat. No. 564175; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Panel or APC Rat Anti-Mouse CD127 antibody Cat. No. 564175; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Panel or APC Rat Anti-Mouse CD127 antibody Cat. No. 564175; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Panel or APC Rat Anti-Mouse CD127 antibody Cat. No. 564175; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Panel or APC Rat Anti-Mouse CD127 antibody Cat. No. 564175; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD127 expression on mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064/553063/561824 and either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Panel or APC Rat Anti-Mouse CD127 antibody Cat. No. 564175; Right Panel . Two-color flow cytometric dot plots showing the correlated expression patterns of CD127 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.