BD PharmingenTM APC Rat Anti-Mouse CD182 (CXCR2)

Description

Multicolor flow cytometric analysis of CD182 CXCR2 expression on mouse bone marrow cells. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 563849/566218 and with either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Plot or APC Rat Anti-Mouse CD182 CXCR2 antibody Cat. No. 568201/568210; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD182 CXCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD182 CXCR2 expression on mouse bone marrow cells. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 563849/566218 and with either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Plot or APC Rat Anti-Mouse CD182 CXCR2 antibody Cat. No. 568201/568210; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD182 CXCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD182 CXCR2 expression on mouse bone marrow cells. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 563849/566218 and with either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Plot or APC Rat Anti-Mouse CD182 CXCR2 antibody Cat. No. 568201/568210; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD182 CXCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD182 CXCR2 expression on mouse bone marrow cells. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 563849/566218 and with either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Plot or APC Rat Anti-Mouse CD182 CXCR2 antibody Cat. No. 568201/568210; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD182 CXCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD182 CXCR2 expression on mouse bone marrow cells. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 563849/566218 and with either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Plot or APC Rat Anti-Mouse CD182 CXCR2 antibody Cat. No. 568201/568210; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD182 CXCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD182 CXCR2 expression on mouse bone marrow cells. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 563849/566218 and with either APC Rat IgG2b, kappa Isotype Control Cat. No. 553991; Left Plot or APC Rat Anti-Mouse CD182 CXCR2 antibody Cat. No. 568201/568210; Right Plot at 1.0 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD182 CXCR2 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.