BD PharmingenTM APC Rat Anti-Mouse CD205 (DEC-205)

Description

Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either APC Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or APC Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567500; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either APC Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or APC Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567500; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either APC Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or APC Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567500; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either APC Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or APC Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567500; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either APC Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or APC Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567500; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD205 DEC-205 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody Cat. No. 562709 and with either APC Rat IgG2a, kappa Isotype Control Cat. No. 553930; Left Plot or APC Rat Anti-Mouse CD205 DEC-205 antibody Cat. No. 567500; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 DEC-205 or Ig Isotype control staining versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.