BD PharmingenTM APC Rat anti-Mouse CD273

Description

Flow cytometric analysis of APC-conjugated anti-mouse CD273 on mouse splenic dendritic cells. Splenic dendritic cells were generated from BALB/c mouse spleen by overnight culture with 5 ng/ml recombinant mouse GM-CSF Cat. no. 554586 . They were stained with APC conjugated anti-mouse CD273 clone TY25, Cat. No. 560086 or an APC conjugated rat IgG2a isotype control clone R35-95, Cat. No. 553932; data not shown in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. no. 553141 . DC were identified by staining with FITC Hamster anti-mouse CD11c mAb HL3 Cat. no. 550261 and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur System and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes. Flow cytometric analysis of APC-conjugated anti-mouse CD273 on mouse splenic dendritic cells. Splenic dendritic cells were generated from BALB/c mouse spleen by overnight culture with 5 ng/ml recombinant mouse GM-CSF Cat. no. 554586 . They were stained with APC conjugated anti-mouse CD273 clone TY25, Cat. No. 560086 or an APC conjugated rat IgG2a isotype control clone R35-95, Cat. No. 553932; data not shown in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. no. 553141 . DC were identified by staining with FITC Hamster anti-mouse CD11c mAb HL3 Cat. no. 550261 and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur System and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes. Show More Flow cytometric analysis of APC-conjugated anti-mouse CD273 on mouse splenic dendritic cells. Splenic dendritic cells were generated from BALB/c mouse spleen by overnight culture with 5 ng/ml recombinant mouse GM-CSF Cat. no. 554586 . They were stained with APC conjugated anti-mouse CD273 clone TY25, Cat. No. 560086 or an APC conjugated rat IgG2a isotype control clone R35-95, Cat. No. 553932; data not shown in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. no. 553141 . DC were identified by staining with FITC Hamster anti-mouse CD11c mAb HL3 Cat. no. 550261 and analyzed by flow cytometry. Flow cytometry was performed on a BD FACSCalibur System and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes.