BD PharmingenTM APC Rat Anti-Mouse CD326

Description

Two-color analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat IgG2a, kappa Isotype Control Cat. No. 554690 or APC Rat Anti-Mouse CD326 antibody Cat. No. 563478 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 and BD Horizon PE-CF594 Rat Anti-Mouse CD25 Cat. No. 562694/562695 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Panel or CD326 Right Panel versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat IgG2a, kappa Isotype Control Cat. No. 554690 or APC Rat Anti-Mouse CD326 antibody Cat. No. 563478 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 and BD Horizon PE-CF594 Rat Anti-Mouse CD25 Cat. No. 562694/562695 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Panel or CD326 Right Panel versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat IgG2a, kappa Isotype Control Cat. No. 554690 or APC Rat Anti-Mouse CD326 antibody Cat. No. 563478 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 and BD Horizon PE-CF594 Rat Anti-Mouse CD25 Cat. No. 562694/562695 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Panel or CD326 Right Panel versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat IgG2a, kappa Isotype Control Cat. No. 554690 or APC Rat Anti-Mouse CD326 antibody Cat. No. 563478 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 and BD Horizon PE-CF594 Rat Anti-Mouse CD25 Cat. No. 562694/562695 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Panel or CD326 Right Panel versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat IgG2a, kappa Isotype Control Cat. No. 554690 or APC Rat Anti-Mouse CD326 antibody Cat. No. 563478 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 and BD Horizon PE-CF594 Rat Anti-Mouse CD25 Cat. No. 562694/562695 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Panel or CD326 Right Panel versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat IgG2a, kappa Isotype Control Cat. No. 554690 or APC Rat Anti-Mouse CD326 antibody Cat. No. 563478 . Left Panel: Thymocytes were further stained with FITC Rat Anti-Mouse CD4 553047/553046/561835 and PE Rat Anti-Mouse CD8a 553033/553032/561095 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Panels: The splenic leucocytes were further stained with PE Hamster Anti-Mouse CD3e Cat. No. 553064/553063/561824 and BD Horizon PE-CF594 Rat Anti-Mouse CD25 Cat. No. 562694/562695 antibodies. Two-color flow cytometric dot plots show the correlated expression patterns of Ig Isotype control staining Middle Panel or CD326 Right Panel versus CD3e for CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells were detected Right Panel , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.