BD PharmingenTM APC Rat Anti-Mouse CD71

Description

Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat anti-Mouse TER-119/Erythroid Cells antibody Cat. No. 563827/566206 and either APC Rat IgG2a, kappa Isotype Control Cat. No. 554690; Left Plot or APC Rat Anti-Mouse CD71 antibody Cat. No. 567258; Right Plot at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression or Ig Isotype control staining versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD Fortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat anti-Mouse TER-119/Erythroid Cells antibody Cat. No. 563827/566206 and either APC Rat IgG2a, kappa Isotype Control Cat. No. 554690; Left Plot or APC Rat Anti-Mouse CD71 antibody Cat. No. 567258; Right Plot at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression or Ig Isotype control staining versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD Fortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat anti-Mouse TER-119/Erythroid Cells antibody Cat. No. 563827/566206 and either APC Rat IgG2a, kappa Isotype Control Cat. No. 554690; Left Plot or APC Rat Anti-Mouse CD71 antibody Cat. No. 567258; Right Plot at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression or Ig Isotype control staining versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD Fortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat anti-Mouse TER-119/Erythroid Cells antibody Cat. No. 563827/566206 and either APC Rat IgG2a, kappa Isotype Control Cat. No. 554690; Left Plot or APC Rat Anti-Mouse CD71 antibody Cat. No. 567258; Right Plot at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression or Ig Isotype control staining versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD Fortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat anti-Mouse TER-119/Erythroid Cells antibody Cat. No. 563827/566206 and either APC Rat IgG2a, kappa Isotype Control Cat. No. 554690; Left Plot or APC Rat Anti-Mouse CD71 antibody Cat. No. 567258; Right Plot at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression or Ig Isotype control staining versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD Fortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD71 expression on mouse bone marrow cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat anti-Mouse TER-119/Erythroid Cells antibody Cat. No. 563827/566206 and either APC Rat IgG2a, kappa Isotype Control Cat. No. 554690; Left Plot or APC Rat Anti-Mouse CD71 antibody Cat. No. 567258; Right Plot at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to the cells right before analysis. The bivariate pseudocolor density plot showing CD71 expression or Ig Isotype control staining versus TER-119 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD Fortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.