BD PharmingenTM APC Rat Anti-Mouse F4/80

Description

Two-color flow cytometric analysis of F4/80 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD11b antibody Cat. No. 553311/557397/561689 and either APC Rat IgG2a Isotype Control Cat. No. 553932; Left Plot or APC Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of F4/80 expression on mouse peritoneal cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD117 Cat. No. 553355/561075 and either APC Rat IgG2a Isotype Control Cat. No. 553932; Left Plot or APC Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The two-color dot plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of F4/80 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD11b antibody Cat. No. 553311/557397/561689 and either APC Rat IgG2a Isotype Control Cat. No. 553932; Left Plot or APC Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of F4/80 expression on mouse peritoneal cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD117 Cat. No. 553355/561075 and either APC Rat IgG2a Isotype Control Cat. No. 553932; Left Plot or APC Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The two-color dot plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of F4/80 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD11b antibody Cat. No. 553311/557397/561689 and either APC Rat IgG2a Isotype Control Cat. No. 553932; Left Plot or APC Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The two-color flow cytometric dot plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of F4/80 expression on mouse peritoneal cells. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with PE Rat Anti-Mouse CD117 Cat. No. 553355/561075 and either APC Rat IgG2a Isotype Control Cat. No. 553932; Left Plot or APC Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The two-color dot plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.