BD PharmingenTM APC Rat Anti-Mouse IgG1

Description

Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody Cat. No. 550874 at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti- Rat CD45RA antibody Cat. No. 554882; solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 mug/test. The fluorescence histogram showing CD45RA expression or control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody Cat. No. 550874 at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti- Rat CD45RA antibody Cat. No. 554882; solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 mug/test. The fluorescence histogram showing CD45RA expression or control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody Cat. No. 550874 at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti- Rat CD45RA antibody Cat. No. 554882; solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 mug/test. The fluorescence histogram showing CD45RA expression or control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody Cat. No. 550874 at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti- Rat CD45RA antibody Cat. No. 554882; solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 mug/test. The fluorescence histogram showing CD45RA expression or control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody Cat. No. 550874 at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti- Rat CD45RA antibody Cat. No. 554882; solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 mug/test. The fluorescence histogram showing CD45RA expression or control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of mouse CD22.2 and rat CD45RA expression using APC Rat Anti-Mouse IgG1 antibody as a second step. Left Plot: CD22.2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti-Mouse CD22.2 antibody solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody Cat. No. 550874 at 1 mug/test. The fluorescence histogram showing CD22.2 expression or control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Plot: CD45RA expression on rat splenocytes. Rat splenic leucocytes were either not stained control staining; dashed line histogram or stained with Purified Mouse IgG1, kappa Anti- Rat CD45RA antibody Cat. No. 554882; solid line histogram . After washing, the cells were then stained with APC Rat Anti-Mouse IgG1 antibody at 1 mug/test. The fluorescence histogram showing CD45RA expression or control staining was derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.