BD PharmingenTM APC Rat Anti-Mouse IL-2

Description

Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/C mice were stimulated for 15 hr with hamster anti-mouse CD3 25 mug/ml, clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml, clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiPlug Cat. No. 555029 . The splenocytes were harvested, stained with 0.06 mug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC-conjugated rat anti-mouse IL-2 antibody APC-JES6-5H4, Cat. No. 554429 by using the BD Pharmingen staining protocol left panel . To demonstrate specificity of staining, the staining by APC-JES6-5H4 was blocked by each of the following: 1 preincubation of the conjugated antibody with recombinant mouse IL-2 0.25 mug, Cat. No. 550069; middle panel and by 2 preincubation of the fixed/permeabilized cells with unlabelled JES6-5H4 mouse antibody 5.0 mug; Cat. No. 554425 prior to staining with the APC-JES6-5H4 right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/C mice were stimulated for 15 hr with hamster anti-mouse CD3 25 mug/ml, clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml, clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiPlug Cat. No. 555029 . The splenocytes were harvested, stained with 0.06 mug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC-conjugated rat anti-mouse IL-2 antibody APC-JES6-5H4, Cat. No. 554429 by using the BD Pharmingen staining protocol left panel . To demonstrate specificity of staining, the staining by APC-JES6-5H4 was blocked by each of the following: 1 preincubation of the conjugated antibody with recombinant mouse IL-2 0.25 mug, Cat. No. 550069; middle panel and by 2 preincubation of the fixed/permeabilized cells with unlabelled JES6-5H4 mouse antibody 5.0 mug; Cat. No. 554425 prior to staining with the APC-JES6-5H4 right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/C mice were stimulated for 15 hr with hamster anti-mouse CD3 25 mug/ml, clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml, clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiPlug Cat. No. 555029 . The splenocytes were harvested, stained with 0.06 mug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC-conjugated rat anti-mouse IL-2 antibody APC-JES6-5H4, Cat. No. 554429 by using the BD Pharmingen staining protocol left panel . To demonstrate specificity of staining, the staining by APC-JES6-5H4 was blocked by each of the following: 1 preincubation of the conjugated antibody with recombinant mouse IL-2 0.25 mug, Cat. No. 550069; middle panel and by 2 preincubation of the fixed/permeabilized cells with unlabelled JES6-5H4 mouse antibody 5.0 mug; Cat. No. 554425 prior to staining with the APC-JES6-5H4 right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Show More Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/C mice were stimulated for 15 hr with hamster anti-mouse CD3 25 mug/ml, clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml, clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiPlug Cat. No. 555029 . The splenocytes were harvested, stained with 0.06 mug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC-conjugated rat anti-mouse IL-2 antibody APC-JES6-5H4, Cat. No. 554429 by using the BD Pharmingen staining protocol left panel . To demonstrate specificity of staining, the staining by APC-JES6-5H4 was blocked by each of the following: 1 preincubation of the conjugated antibody with recombinant mouse IL-2 0.25 mug, Cat. No. 550069; middle panel and by 2 preincubation of the fixed/permeabilized cells with unlabelled JES6-5H4 mouse antibody 5.0 mug; Cat. No. 554425 prior to staining with the APC-JES6-5H4 right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Show More Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/C mice were stimulated for 15 hr with hamster anti-mouse CD3 25 mug/ml, clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml, clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiPlug Cat. No. 555029 . The splenocytes were harvested, stained with 0.06 mug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC-conjugated rat anti-mouse IL-2 antibody APC-JES6-5H4, Cat. No. 554429 by using the BD Pharmingen staining protocol left panel . To demonstrate specificity of staining, the staining by APC-JES6-5H4 was blocked by each of the following: 1 preincubation of the conjugated antibody with recombinant mouse IL-2 0.25 mug, Cat. No. 550069; middle panel and by 2 preincubation of the fixed/permeabilized cells with unlabelled JES6-5H4 mouse antibody 5.0 mug; Cat. No. 554425 prior to staining with the APC-JES6-5H4 right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/C mice were stimulated for 15 hr with hamster anti-mouse CD3 25 mug/ml, clone 145-2C11, Cat. No. 553057 and hamster anti-mouse CD28 2 mug/ml, clone 37.51, Cat. No. 553294 antibodies in the presence of BD GolgiPlug Cat. No. 555029 . The splenocytes were harvested, stained with 0.06 mug of FITC-conjugated rat anti-mouse CD4 FITC-RM4-5, Cat. No. 553047 , fixed, permeabilized, and subsequently stained with 0.25 mug of APC-conjugated rat anti-mouse IL-2 antibody APC-JES6-5H4, Cat. No. 554429 by using the BD Pharmingen staining protocol left panel . To demonstrate specificity of staining, the staining by APC-JES6-5H4 was blocked by each of the following: 1 preincubation of the conjugated antibody with recombinant mouse IL-2 0.25 mug, Cat. No. 550069; middle panel and by 2 preincubation of the fixed/permeabilized cells with unlabelled JES6-5H4 mouse antibody 5.0 mug; Cat. No. 554425 prior to staining with the APC-JES6-5H4 right panel . The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls. This APC-conjugated reagent can be used in any flow cytometer equipped with a a dye, HeNE or red diode laser. These include the dual laser FACStarPLUS, FACS Vantage or FACSCalibur.