BD PharmingenTM APO-DIRECTTM Kit

Description

Schematic Representation of APO-DIRECT Labeling. The enzyme terminal deoxynucleotidyl transferase TdT catalyzes a template independent addition of FITC-labeled deoxyuridine triphosphates FITC-dUTP to the 3-hydroxyl ends of double- and single-stranded DNA. After Br-dUTP incorporation, DNA break sites are identified by the FITC-labeled dUTPs. Flow Cytometry Data of APO-DIRECT Negative and Positive Control Cells. Typical results using the positive and negative control cells are shown in this figure. Negative control cells top row or positive control cells bottom row are treated with FITC-dUTP in the presence of TdT enzyme which incorporates FITC-dUTP into the 3-hydroxyl-DNA ends found in apoptotic cells. Cells are then stained with propidium iodide and analyzed on a Becton Dickinson FACScan. The presence of apoptotic cells in the right hand panel bottom row is demonstrated by increased fluorescence intensity M2 gate . Propidium iodide staining panel insets provides additional cell cycle information about each population, indicating that the majority of apoptotic cells are in S-G2 phases of the cell cycle. Flow Cytometer Setup for Becton Dickinson Hardware. Cells are labeled with both PI DNA and FITC Apoptotic Cells . Display 1: Non-clumped cells are gated. Gated from Display 1: A circle is drawn around cells Schematic Representation of APO-DIRECT Labeling. The enzyme terminal deoxynucleotidyl transferase TdT catalyzes a template independent addition of FITC-labeled deoxyuridine triphosphates FITC-dUTP to the 3-hydroxyl ends of double- and single-stranded DNA. After Br-dUTP incorporation, DNA break sites are identified by the FITC-labeled dUTPs. Show More Flow Cytometry Data of APO-DIRECT Negative and Positive Control Cells. Typical results using the positive and negative control cells are shown in this figure. Negative control cells top row or positive control cells bottom row are treated with FITC-dUTP in the presence of TdT enzyme which incorporates FITC-dUTP into the 3-hydroxyl-DNA ends found in apoptotic cells. Cells are then stained with propidium iodide and analyzed on a Becton Dickinson FACScan. The presence of apoptotic cells in the right hand panel bottom row is demonstrated by increased fluorescence intensity M2 gate . Propidium iodide staining panel insets provides additional cell cycle information about each population, indicating that the majority of apoptotic cells are in S-G2 phases of the cell cycle. Show More Flow Cytometer Setup for Becton Dickinson Hardware. Cells are labeled with both PI DNA and FITC Apoptotic Cells . Display 1: Non-clumped cells are gated. Gated from Display 1: A circle is drawn around cells Show More Schematic Representation of APO-DIRECT Labeling. The enzyme terminal deoxynucleotidyl transferase TdT catalyzes a template independent addition of FITC-labeled deoxyuridine triphosphates FITC-dUTP to the 3-hydroxyl ends of double- and single-stranded DNA. After Br-dUTP incorporation, DNA break sites are identified by the FITC-labeled dUTPs. Flow Cytometry Data of APO-DIRECT Negative and Positive Control Cells. Typical results using the positive and negative control cells are shown in this figure. Negative control cells top row or positive control cells bottom row are treated with FITC-dUTP in the presence of TdT enzyme which incorporates FITC-dUTP into the 3-hydroxyl-DNA ends found in apoptotic cells. Cells are then stained with propidium iodide and analyzed on a Becton Dickinson FACScan. The presence of apoptotic cells in the right hand panel bottom row is demonstrated by increased fluorescence intensity M2 gate . Propidium iodide staining panel insets provides additional cell cycle information about each population, indicating that the majority of apoptotic cells are in S-G2 phases of the cell cycle. Flow Cytometer Setup for Becton Dickinson Hardware. Cells are labeled with both PI DNA and FITC Apoptotic Cells . Display 1: Non-clumped cells are gated. Gated from Display 1: A circle is drawn around cells