BD PharmingenTM Biotin Annexin V

Description

Biotin Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated upper left & lower left panels , treated for 5 hours upper middle & lower middle panels or 12 hours upper right & lower right panels with anti-human Fas antibody clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with Biotin Annexin V, followed by incubation with SAv-FITC in a buffer containing Propidium Iodide PI . Cells were then analyzed by flow cytometry. Untreated cells were primarily Biotin Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. After a 5 hr treatment with DX2, there were two populations of cells: Cells undergoing apoptosis Biotin Annexin V positive and PI negative , and cells that were viable and not undergoing apoptosis Biotin Annexin V and PI negative . After a 12 hr treatment with DX2, three populations of cells were identified: Cells that had already died or were in late stage of apoptosis Biotin Annexin V and PI positive , cells undergoing apoptosis Biotin Annexin V positive and PI negative , and cells that were viable and not undergoing apoptosis Biotin Annexin V and PI negative . The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by cross-linking the Fas receptor. Biotin Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated upper left & lower left panels , treated for 5 hours upper middle & lower middle panels or 12 hours upper right & lower right panels with anti-human Fas antibody clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with Biotin Annexin V, followed by incubation with SAv-FITC in a buffer containing Propidium Iodide PI . Cells were then analyzed by flow cytometry. Untreated cells were primarily Biotin Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. After a 5 hr treatment with DX2, there were two populations of cells: Cells undergoing apoptosis Biotin Annexin V positive and PI negative , and cells that were viable and not undergoing apoptosis Biotin Annexin V and PI negative . After a 12 hr treatment with DX2, three populations of cells were identified: Cells that had already died or were in late stage of apoptosis Biotin Annexin V and PI positive , cells undergoing apoptosis Biotin Annexin V positive and PI negative , and cells that were viable and not undergoing apoptosis Biotin Annexin V and PI negative . The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by cross-linking the Fas receptor. Show More Biotin Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated upper left & lower left panels , treated for 5 hours upper middle & lower middle panels or 12 hours upper right & lower right panels with anti-human Fas antibody clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with Biotin Annexin V, followed by incubation with SAv-FITC in a buffer containing Propidium Iodide PI . Cells were then analyzed by flow cytometry. Untreated cells were primarily Biotin Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. After a 5 hr treatment with DX2, there were two populations of cells: Cells undergoing apoptosis Biotin Annexin V positive and PI negative , and cells that were viable and not undergoing apoptosis Biotin Annexin V and PI negative . After a 12 hr treatment with DX2, three populations of cells were identified: Cells that had already died or were in late stage of apoptosis Biotin Annexin V and PI positive , cells undergoing apoptosis Biotin Annexin V positive and PI negative , and cells that were viable and not undergoing apoptosis Biotin Annexin V and PI negative . The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by cross-linking the Fas receptor.