BD PharmingenTM Biotin Hamster Anti-Mouse CD28

Description

Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 , in addition to staining with biotinylated anti-mouse CD28 clone 37.51 right panel , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 , in addition to staining with biotinylated anti-mouse CD28 clone 37.51 right panel , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 , in addition to staining with biotinylated anti-mouse CD28 clone 37.51 right panel , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Show More Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 , in addition to staining with biotinylated anti-mouse CD28 clone 37.51 right panel , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Show More Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 , in addition to staining with biotinylated anti-mouse CD28 clone 37.51 right panel , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system. Two-color analysis of CD28 expression on splenic T lymphocytes. After pre-incubation with purified anti-mouse CD16/CD32 mAb 2.4G2 Mouse BD Fc Block Cat. No. 553141 , BALB/c splenocytes were simultaneously stained with FITC-conjugated anti-mouse CD4 mAb RM4-5 Cat. No. 553046 and FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030 , in addition to staining with biotinylated anti-mouse CD28 clone 37.51 right panel , followed by streptavidin-PE Cat. No. 554061 . Flow cytometry was performed on a BD FACScan flow cytometry system.