BD PharmingenTM Biotin Mouse Anti-GFP

Description

Figure 1. Flow cytometric analysis of GFP expression in transfected human embryonic kidney cells. AcGFP-transfected HEK-293 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with the BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with either Biotin Mouse IgG1 kappa Isotype Control dashed line, Cat. No. 555747 or Biotin Mouse Anti-GFP monoclonal antibody solid line, Cat. No. 565271 followed by APC Streptavidin Cat. No. 554067 . The histograms were derived from gated events based on light scattering characteristics of intact AcGFP-transfected HEK-293 cells. Flow cytometry was performed on a BD LSRFortessa Cell Analyzer System. Figure 2. Immunofluorescent staining of GFP-transfected human embryonic kidney cells. Untransfected HEK-293 cells left panel and AcGFP-transfected HEK-293 cells middle and right panels were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with 0.1 % Triton X-100. The cells were blocked with Avidin D followed by Biotin Vector Labs , and then stained with Biotin Mouse Anti-GFP monoclonal antibody left and middle panels, pseudo-colored red at 20 mug/mL. The secondary reagent was Streptavidin Alexa Fluor 555 Life Technologies at 5 mug/mL. GFP signal in the AcGFP-transfected HEK-293 cells is shown for comparison right panel, pseudo-colored green . Cell nuclei were counterstained with DAPI Cat. No. 564907 . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Figure 1. Flow cytometric analysis of GFP expression in transfected human embryonic kidney cells. AcGFP-transfected HEK-293 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with the BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with either Biotin Mouse IgG1 kappa Isotype Control dashed line, Cat. No. 555747 or Biotin Mouse Anti-GFP monoclonal antibody solid line, Cat. No. 565271 followed by APC Streptavidin Cat. No. 554067 . The histograms were derived from gated events based on light scattering characteristics of intact AcGFP-transfected HEK-293 cells. Flow cytometry was performed on a BD LSRFortessa Cell Analyzer System. Figure 2. Immunofluorescent staining of GFP-transfected human embryonic kidney cells. Untransfected HEK-293 cells left panel and AcGFP-transfected HEK-293 cells middle and right panels were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with 0.1 % Triton X-100. The cells were blocked with Avidin D followed by Biotin Vector Labs , and then stained with Biotin Mouse Anti-GFP monoclonal antibody left and middle panels, pseudo-colored red at 20 mug/mL. The secondary reagent was Streptavidin Alexa Fluor 555 Life Technologies at 5 mug/mL. GFP signal in the AcGFP-transfected HEK-293 cells is shown for comparison right panel, pseudo-colored green . Cell nuclei were counterstained with DAPI Cat. No. 564907 . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Show More Figure 1. Flow cytometric analysis of GFP expression in transfected human embryonic kidney cells. AcGFP-transfected HEK-293 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with the BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with either Biotin Mouse IgG1 kappa Isotype Control dashed line, Cat. No. 555747 or Biotin Mouse Anti-GFP monoclonal antibody solid line, Cat. No. 565271 followed by APC Streptavidin Cat. No. 554067 . The histograms were derived from gated events based on light scattering characteristics of intact AcGFP-transfected HEK-293 cells. Flow cytometry was performed on a BD LSRFortessa Cell Analyzer System. Figure 2. Immunofluorescent staining of GFP-transfected human embryonic kidney cells. Untransfected HEK-293 cells left panel and AcGFP-transfected HEK-293 cells middle and right panels were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with 0.1 % Triton X-100. The cells were blocked with Avidin D followed by Biotin Vector Labs , and then stained with Biotin Mouse Anti-GFP monoclonal antibody left and middle panels, pseudo-colored red at 20 mug/mL. The secondary reagent was Streptavidin Alexa Fluor 555 Life Technologies at 5 mug/mL. GFP signal in the AcGFP-transfected HEK-293 cells is shown for comparison right panel, pseudo-colored green . Cell nuclei were counterstained with DAPI Cat. No. 564907 . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software.