BD PharmingenTM Biotin Mouse Anti-Human CD120a

Description

Flow cytometric analysis of expression of cell surface TNFRI by whole-lysed human blood. Whole human blood was stained with Biotin Mouse Anti-Human CD120a Cat No. 550900 followed by PE Streptavidin Cat. No. 554061 . Red blood cells were subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with the Biotin Mouse Anti-Human CD120a filled histogram is compared to staining obtained using the Biotin Mouse IgG2a, kappa Isotype Control Cat. No. 553455; open histograms . Histograms in figure are gated on the lymphocyte left panel , monocyte middle panel and granulocyte right panel populations based on the side and forward light scattering characteristics. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Reference by Browning et. al. . Flow cytometric analysis of expression of cell surface TNFRI by whole-lysed human blood. Whole human blood was stained with Biotin Mouse Anti-Human CD120a Cat No. 550900 followed by PE Streptavidin Cat. No. 554061 . Red blood cells were subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with the Biotin Mouse Anti-Human CD120a filled histogram is compared to staining obtained using the Biotin Mouse IgG2a, kappa Isotype Control Cat. No. 553455; open histograms . Histograms in figure are gated on the lymphocyte left panel , monocyte middle panel and granulocyte right panel populations based on the side and forward light scattering characteristics. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Reference by Browning et. al. . Flow cytometric analysis of expression of cell surface TNFRI by whole-lysed human blood. Whole human blood was stained with Biotin Mouse Anti-Human CD120a Cat No. 550900 followed by PE Streptavidin Cat. No. 554061 . Red blood cells were subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with the Biotin Mouse Anti-Human CD120a filled histogram is compared to staining obtained using the Biotin Mouse IgG2a, kappa Isotype Control Cat. No. 553455; open histograms . Histograms in figure are gated on the lymphocyte left panel , monocyte middle panel and granulocyte right panel populations based on the side and forward light scattering characteristics. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Reference by Browning et. al. . Show More Flow cytometric analysis of expression of cell surface TNFRI by whole-lysed human blood. Whole human blood was stained with Biotin Mouse Anti-Human CD120a Cat No. 550900 followed by PE Streptavidin Cat. No. 554061 . Red blood cells were subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with the Biotin Mouse Anti-Human CD120a filled histogram is compared to staining obtained using the Biotin Mouse IgG2a, kappa Isotype Control Cat. No. 553455; open histograms . Histograms in figure are gated on the lymphocyte left panel , monocyte middle panel and granulocyte right panel populations based on the side and forward light scattering characteristics. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Reference by Browning et. al. . Show More Flow cytometric analysis of expression of cell surface TNFRI by whole-lysed human blood. Whole human blood was stained with Biotin Mouse Anti-Human CD120a Cat No. 550900 followed by PE Streptavidin Cat. No. 554061 . Red blood cells were subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with the Biotin Mouse Anti-Human CD120a filled histogram is compared to staining obtained using the Biotin Mouse IgG2a, kappa Isotype Control Cat. No. 553455; open histograms . Histograms in figure are gated on the lymphocyte left panel , monocyte middle panel and granulocyte right panel populations based on the side and forward light scattering characteristics. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Reference by Browning et. al. . Flow cytometric analysis of expression of cell surface TNFRI by whole-lysed human blood. Whole human blood was stained with Biotin Mouse Anti-Human CD120a Cat No. 550900 followed by PE Streptavidin Cat. No. 554061 . Red blood cells were subsequently lysed with Lysing Buffer Cat No. 555899 . Staining with the Biotin Mouse Anti-Human CD120a filled histogram is compared to staining obtained using the Biotin Mouse IgG2a, kappa Isotype Control Cat. No. 553455; open histograms . Histograms in figure are gated on the lymphocyte left panel , monocyte middle panel and granulocyte right panel populations based on the side and forward light scattering characteristics. Note: Certain human cell lines or cell types e.g., neutrophils, monocytes can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors for example see Reference by Browning et. al. .