
567709 ·
BDB Bioscience · Cat: 567709
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of CD20 expression on human peripheral blood leucocyte populations. Whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415/555741; Left Plot or Biotin Mouse Anti-Human CD20 antibody Cat. No. 567709; Right Plot at 0.5 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD20 expression on human peripheral blood leucocyte populations. Whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415/555741; Left Plot or Biotin Mouse Anti-Human CD20 antibody Cat. No. 567709; Right Plot at 0.5 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD20 expression on human peripheral blood leucocyte populations. Whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415/555741; Left Plot or Biotin Mouse Anti-Human CD20 antibody Cat. No. 567709; Right Plot at 0.5 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD20 expression on human peripheral blood leucocyte populations. Whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415/555741; Left Plot or Biotin Mouse Anti-Human CD20 antibody Cat. No. 567709; Right Plot at 0.5 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD20 expression on human peripheral blood leucocyte populations. Whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415/555741; Left Plot or Biotin Mouse Anti-Human CD20 antibody Cat. No. 567709; Right Plot at 0.5 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD20 expression on human peripheral blood leucocyte populations. Whole blood was stained with either Biotin Mouse IgG2b, kappa Isotype Control Cat. No. 565415/555741; Left Plot or Biotin Mouse Anti-Human CD20 antibody Cat. No. 567709; Right Plot at 0.5 mug/test. The cells were counterstained with PE-Streptavidin Cat. No. 554061 . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.