BD PharmingenTM Biotin Mouse Anti-Human CD269 (BCMA)

Description

Flow cytometric analysis of CD269 BCMA expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Biotin Mouse IgG1, kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD269 BCMA antibody Cat. No. 571225; solid line histograms followed by BD Pharmingen PE Streptavidin Cat. No. 554061 at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD269 BCMA expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD269 BCMA expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Biotin Mouse IgG1, kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD269 BCMA antibody Cat. No. 571225; solid line histograms followed by BD Pharmingen PE Streptavidin Cat. No. 554061 at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD269 BCMA expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD269 BCMA expression on Human U266 cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Biotin Mouse IgG1, kappa Isotype Control Cat. No. 555747; dashed line histogram or Biotin Mouse Anti-Human CD269 BCMA antibody Cat. No. 571225; solid line histograms followed by BD Pharmingen PE Streptavidin Cat. No. 554061 at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD269 BCMA expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.