BD PharmingenTM Biotin Mouse Anti-Human CD57

Description

Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat. No.561811 and with either Biotin Mouse IgM, kappa Isotype Control Cat. No. 553473; Left Plot or Biotin Mouse Anti-Human CD57 antibody Cat. No. 568064; Right Plot at 1 mug/test. The cells were washed and counterstained with PE Strptavidin Cat. No. 554061 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD57 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat. No.561811 and with either Biotin Mouse IgM, kappa Isotype Control Cat. No. 553473; Left Plot or Biotin Mouse Anti-Human CD57 antibody Cat. No. 568064; Right Plot at 1 mug/test. The cells were washed and counterstained with PE Strptavidin Cat. No. 554061 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD57 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat. No.561811 and with either Biotin Mouse IgM, kappa Isotype Control Cat. No. 553473; Left Plot or Biotin Mouse Anti-Human CD57 antibody Cat. No. 568064; Right Plot at 1 mug/test. The cells were washed and counterstained with PE Strptavidin Cat. No. 554061 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD57 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat. No.561811 and with either Biotin Mouse IgM, kappa Isotype Control Cat. No. 553473; Left Plot or Biotin Mouse Anti-Human CD57 antibody Cat. No. 568064; Right Plot at 1 mug/test. The cells were washed and counterstained with PE Strptavidin Cat. No. 554061 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD57 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat. No.561811 and with either Biotin Mouse IgM, kappa Isotype Control Cat. No. 553473; Left Plot or Biotin Mouse Anti-Human CD57 antibody Cat. No. 568064; Right Plot at 1 mug/test. The cells were washed and counterstained with PE Strptavidin Cat. No. 554061 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD57 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD3 antibody Cat. No.561811 and with either Biotin Mouse IgM, kappa Isotype Control Cat. No. 553473; Left Plot or Biotin Mouse Anti-Human CD57 antibody Cat. No. 568064; Right Plot at 1 mug/test. The cells were washed and counterstained with PE Strptavidin Cat. No. 554061 . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD57 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.