BD PharmingenTM Biotin Mouse Anti-Mouse Fcmu Receptor

Description

Two-color flow cytometric analysis of Fcmu Receptor expression on mouse splenic B cells. C57BL/6 Upper Plots or BALB/c Lower Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992/561738 and either Biotin Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plots or Biotin Mouse Anti-Mouse Fcmu Receptor antibody Cat. No. 566837; Right Plots at 2 mug/test. The cells were washed and then counterstained with PE Streptavidin Cat. No. 554061 . Two-color flow cytometric contour plots showing the correlated expression of Fcmu Receptor or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Fcmu Receptor expression on mouse splenic B cells. C57BL/6 Upper Plots or BALB/c Lower Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992/561738 and either Biotin Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plots or Biotin Mouse Anti-Mouse Fcmu Receptor antibody Cat. No. 566837; Right Plots at 2 mug/test. The cells were washed and then counterstained with PE Streptavidin Cat. No. 554061 . Two-color flow cytometric contour plots showing the correlated expression of Fcmu Receptor or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Fcmu Receptor expression on mouse splenic B cells. C57BL/6 Upper Plots or BALB/c Lower Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992/561738 and either Biotin Mouse IgG1, kappa Isotype Control Cat. No. 554680; Left Plots or Biotin Mouse Anti-Mouse Fcmu Receptor antibody Cat. No. 566837; Right Plots at 2 mug/test. The cells were washed and then counterstained with PE Streptavidin Cat. No. 554061 . Two-color flow cytometric contour plots showing the correlated expression of Fcmu Receptor or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.