BD PharmingenTM Biotin Mouse Anti-Pig CD16

Description

Two-color analysis of CD16 expression on pig peripheral blood lymphocytes . Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 Cat. no. 559586, both panels and biotinylated mAb FcG7 Right panel , followed by Streptavidin- FITC Cat. No. 554060, both panels . Erythrocytes were lysed PharmLyse Lysing Buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD Via- Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura BDIS, San Jose, CA . Two-color analysis of CD16 expression on pig peripheral blood lymphocytes . Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 Cat. no. 559586, both panels and biotinylated mAb FcG7 Right panel , followed by Streptavidin- FITC Cat. No. 554060, both panels . Erythrocytes were lysed PharmLyse Lysing Buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD Via- Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura BDIS, San Jose, CA . Two-color analysis of CD16 expression on pig peripheral blood lymphocytes . Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 Cat. no. 559586, both panels and biotinylated mAb FcG7 Right panel , followed by Streptavidin- FITC Cat. No. 554060, both panels . Erythrocytes were lysed PharmLyse Lysing Buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD Via- Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura BDIS, San Jose, CA . Show More Two-color analysis of CD16 expression on pig peripheral blood lymphocytes . Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 Cat. no. 559586, both panels and biotinylated mAb FcG7 Right panel , followed by Streptavidin- FITC Cat. No. 554060, both panels . Erythrocytes were lysed PharmLyse Lysing Buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD Via- Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura BDIS, San Jose, CA . Show More Two-color analysis of CD16 expression on pig peripheral blood lymphocytes . Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 Cat. no. 559586, both panels and biotinylated mAb FcG7 Right panel , followed by Streptavidin- FITC Cat. No. 554060, both panels . Erythrocytes were lysed PharmLyse Lysing Buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD Via- Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura BDIS, San Jose, CA . Two-color analysis of CD16 expression on pig peripheral blood lymphocytes . Pig whole blood was stained with PE-conjugated anti- pig CD4a mAb 74-12-4 Cat. no. 559586, both panels and biotinylated mAb FcG7 Right panel , followed by Streptavidin- FITC Cat. No. 554060, both panels . Erythrocytes were lysed PharmLyse Lysing Buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD Via- Probe, Cat. No. 555816/555815 , and lymphocytes were gated according to their light-scatter profiles. Flow cytometry was performed on a FACSCalibura BDIS, San Jose, CA .