BD PharmingenTM Biotin Mouse Lineage Panel

Description

Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD Mouse Biotin Lineage Panel Cat. No. 559971 and BD IMag Streptavidin Particles Plus - MSC Cat. No. 557812, at 10 mul per 1 x 107 total cells . The labeled cells were then separated over a magnetic separation column designed for depletions. To demonstrate the efficiency of the depletion, unmanipulated bone-marrow cells and the lineage-negative fraction were stained with APC Rat Anti-Mouse CD117 Cat. No. 553356 to detect hematopoietic progenitors, and with PE Rat Anti-Mouse TER-119/Erythroid Cells Cat. No. 553673 , CD45R/B220 Cat. No. 553089/553090 , and CD11b Cat. No. 557397/553311 to detect lineage-committed cells. The lineage-negative fraction contains a greatly increased proportion of CD117+ cells and less than 5 % of lineage-positive contaminants. Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD Mouse Biotin Lineage Panel Cat. No. 559971 and BD IMag Streptavidin Particles Plus - MSC Cat. No. 557812, at 10 mul per 1 x 107 total cells . The labeled cells were then separated over a magnetic separation column designed for depletions. To demonstrate the efficiency of the depletion, unmanipulated bone-marrow cells and the lineage-negative fraction were stained with APC Rat Anti-Mouse CD117 Cat. No. 553356 to detect hematopoietic progenitors, and with PE Rat Anti-Mouse TER-119/Erythroid Cells Cat. No. 553673 , CD45R/B220 Cat. No. 553089/553090 , and CD11b Cat. No. 557397/553311 to detect lineage-committed cells. The lineage-negative fraction contains a greatly increased proportion of CD117+ cells and less than 5 % of lineage-positive contaminants. Show More Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD Mouse Biotin Lineage Panel Cat. No. 559971 and BD IMag Streptavidin Particles Plus - MSC Cat. No. 557812, at 10 mul per 1 x 107 total cells . The labeled cells were then separated over a magnetic separation column designed for depletions. To demonstrate the efficiency of the depletion, unmanipulated bone-marrow cells and the lineage-negative fraction were stained with APC Rat Anti-Mouse CD117 Cat. No. 553356 to detect hematopoietic progenitors, and with PE Rat Anti-Mouse TER-119/Erythroid Cells Cat. No. 553673 , CD45R/B220 Cat. No. 553089/553090 , and CD11b Cat. No. 557397/553311 to detect lineage-committed cells. The lineage-negative fraction contains a greatly increased proportion of CD117+ cells and less than 5 % of lineage-positive contaminants.