BD PharmingenTM Biotin Rabbit Anti- Active Caspase-3

Description

Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or treated with 4 muM of camptothecin for 4 hr to induce apoptosis right panel . Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 for 20 min at room temperature RT , pelleted and washed with BD Perm/Wash buffer component of Cat. No. 554714 . Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody clone C92-605 . Cells were then washed and resuspended in BD Perm/Wash buffer containing FITC-Avidin 5 ng/1x10e6 cells Cat. No. 554057 and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 left panel, M1 ; whereas more than half of the treated cells were positive for active caspase-3 staining right panel, M2 . Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or treated with 4 muM of camptothecin for 4 hr to induce apoptosis right panel . Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 for 20 min at room temperature RT , pelleted and washed with BD Perm/Wash buffer component of Cat. No. 554714 . Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody clone C92-605 . Cells were then washed and resuspended in BD Perm/Wash buffer containing FITC-Avidin 5 ng/1x10e6 cells Cat. No. 554057 and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 left panel, M1 ; whereas more than half of the treated cells were positive for active caspase-3 staining right panel, M2 . Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or treated with 4 muM of camptothecin for 4 hr to induce apoptosis right panel . Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 for 20 min at room temperature RT , pelleted and washed with BD Perm/Wash buffer component of Cat. No. 554714 . Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody clone C92-605 . Cells were then washed and resuspended in BD Perm/Wash buffer containing FITC-Avidin 5 ng/1x10e6 cells Cat. No. 554057 and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 left panel, M1 ; whereas more than half of the treated cells were positive for active caspase-3 staining right panel, M2 . Show More Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or treated with 4 muM of camptothecin for 4 hr to induce apoptosis right panel . Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 for 20 min at room temperature RT , pelleted and washed with BD Perm/Wash buffer component of Cat. No. 554714 . Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody clone C92-605 . Cells were then washed and resuspended in BD Perm/Wash buffer containing FITC-Avidin 5 ng/1x10e6 cells Cat. No. 554057 and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 left panel, M1 ; whereas more than half of the treated cells were positive for active caspase-3 staining right panel, M2 . Show More Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or treated with 4 muM of camptothecin for 4 hr to induce apoptosis right panel . Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 for 20 min at room temperature RT , pelleted and washed with BD Perm/Wash buffer component of Cat. No. 554714 . Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody clone C92-605 . Cells were then washed and resuspended in BD Perm/Wash buffer containing FITC-Avidin 5 ng/1x10e6 cells Cat. No. 554057 and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 left panel, M1 ; whereas more than half of the treated cells were positive for active caspase-3 staining right panel, M2 . Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells Human T-cell leukemia; ATCC TIB-152 were left untreated left panel or treated with 4 muM of camptothecin for 4 hr to induce apoptosis right panel . Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 for 20 min at room temperature RT , pelleted and washed with BD Perm/Wash buffer component of Cat. No. 554714 . Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody clone C92-605 . Cells were then washed and resuspended in BD Perm/Wash buffer containing FITC-Avidin 5 ng/1x10e6 cells Cat. No. 554057 and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 left panel, M1 ; whereas more than half of the treated cells were positive for active caspase-3 staining right panel, M2 .