BD PharmingenTM Biotin Rat Anti-Mouse CD124

Description

Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 0.125 mug/106 cells followed by streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 and FITC anti-mouse B220 0.06 mug, Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the biotinylated mIL4R-M1 antibody right panel is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody 0.125 mug, Cat. No. 553928 that is shown in the left panel. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 0.125 mug/106 cells followed by streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 and FITC anti-mouse B220 0.06 mug, Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the biotinylated mIL4R-M1 antibody right panel is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody 0.125 mug, Cat. No. 553928 that is shown in the left panel. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 0.125 mug/106 cells followed by streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 and FITC anti-mouse B220 0.06 mug, Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the biotinylated mIL4R-M1 antibody right panel is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody 0.125 mug, Cat. No. 553928 that is shown in the left panel. Show More Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 0.125 mug/106 cells followed by streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 and FITC anti-mouse B220 0.06 mug, Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the biotinylated mIL4R-M1 antibody right panel is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody 0.125 mug, Cat. No. 553928 that is shown in the left panel. Show More Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 0.125 mug/106 cells followed by streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 and FITC anti-mouse B220 0.06 mug, Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the biotinylated mIL4R-M1 antibody right panel is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody 0.125 mug, Cat. No. 553928 that is shown in the left panel. Expression of cell surface CD124 by B220-positve and -negative splenic lymphocytes from C57BL/6 mice. Spleen cells from C57BL/6 mice were treated with ACK lysis buffer, washed, and were labeled with purified mouse BD Fc Block Cat. No. 553142 to block mouse Fc receptors. The cells were then stained with biotinylated mIL4R-M1 0.125 mug/106 cells followed by streptavidin phycoerythrin 0.015 mug, Cat. No. 554061 and FITC anti-mouse B220 0.06 mug, Cat. No. 553088 and were analyzed by two-color flow cytometry. The levels of CD124 expressed by B220-positive and B220-negative cells with the light-scattering characteristics of viable lymphocytes are shown in the two-color dot plot right panel . Staining with the biotinylated mIL4R-M1 antibody right panel is compared to staining derived with a biotinylated rat IgG2a immunoglobulin isotype control antibody 0.125 mug, Cat. No. 553928 that is shown in the left panel.