BD PharmingenTM Biotin Rat Anti-Mouse CD45R/B220

Description

Multicolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; Right Plot at 0.25 mug/test, followed by FITC Streptavidin Cat. No. 554060 . The bivariate pseudocolor density plot showing the correlated expression of CD45R/B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; Right Plot at 0.25 mug/test, followed by FITC Streptavidin Cat. No. 554060 . The bivariate pseudocolor density plot showing the correlated expression of CD45R/B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; Right Plot at 0.25 mug/test, followed by FITC Streptavidin Cat. No. 554060 . The bivariate pseudocolor density plot showing the correlated expression of CD45R/B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; Right Plot at 0.25 mug/test, followed by FITC Streptavidin Cat. No. 554060 . The bivariate pseudocolor density plot showing the correlated expression of CD45R/B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; Right Plot at 0.25 mug/test, followed by FITC Streptavidin Cat. No. 554060 . The bivariate pseudocolor density plot showing the correlated expression of CD45R/B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R/B220 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086; Right Plot at 0.25 mug/test, followed by FITC Streptavidin Cat. No. 554060 . The bivariate pseudocolor density plot showing the correlated expression of CD45R/B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.