BD PharmingenTM Biotin Rat Anti-Mouse F4/80

Description

Two-color flow cytometric analysis of F4/80 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BUV395 Rat Anti-Mouse CD11b antibody Cat. No. 563553/565976 and with either Biotin Rat IgG2a Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.06 mug/test. The cells were washed and counterstained with PE Streptavidin Cat. No. 554061 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of F4/80 expression on mouse peritoneal cells. C57BL/6 mouse peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat Anti-Mouse CD117 antibody Cat. No. 562609/566290 and with either Biotin Rat IgG2a Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.06 mug/test. The cells were washed and counterstained with PE Streptavidin Cat. No. 554061 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of F4/80 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BUV395 Rat Anti-Mouse CD11b antibody Cat. No. 563553/565976 and with either Biotin Rat IgG2a Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.06 mug/test. The cells were washed and counterstained with PE Streptavidin Cat. No. 554061 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of F4/80 expression on mouse peritoneal cells. C57BL/6 mouse peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat Anti-Mouse CD117 antibody Cat. No. 562609/566290 and with either Biotin Rat IgG2a Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.06 mug/test. The cells were washed and counterstained with PE Streptavidin Cat. No. 554061 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of F4/80 expression on mouse splenocytes. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BUV395 Rat Anti-Mouse CD11b antibody Cat. No. 563553/565976 and with either Biotin Rat IgG2a Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.06 mug/test. The cells were washed and counterstained with PE Streptavidin Cat. No. 554061 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of F4/80 expression on mouse peritoneal cells. C57BL/6 mouse peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with BD Horizon BV421 Rat Anti-Mouse CD117 antibody Cat. No. 562609/566290 and with either Biotin Rat IgG2a Isotype Control Cat. No. 553928; Left Plot or Biotin Rat Anti-Mouse F4/80 antibody Cat. No. 566787; Right Plot at 0.06 mug/test. The cells were washed and counterstained with PE Streptavidin Cat. No. 554061 . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of F4/80 or Ig Isotype control staining versus CD117 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peritoneal cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are not lot specific.