BD PharmingenTM Biotin Rat Anti-Mouse Pre-B Cell Receptor

Description

Two-color analysis of Pre-B cell receptor Pre-BCR expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM b mAb AF6-78 Cat. No. 553519 , C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553928, left panel or biotinylated mAb SL156 Cat. No. 551863, right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 , followed by Streptavidin-APC Cat. No. 554067 and FITC-conjugated anti-mouse CD45R/B220 mAb Cat. No. 553087/553088 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Two-color analysis of Pre-B cell receptor Pre-BCR expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM b mAb AF6-78 Cat. No. 553519 , C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553928, left panel or biotinylated mAb SL156 Cat. No. 551863, right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 , followed by Streptavidin-APC Cat. No. 554067 and FITC-conjugated anti-mouse CD45R/B220 mAb Cat. No. 553087/553088 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Two-color analysis of Pre-B cell receptor Pre-BCR expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM b mAb AF6-78 Cat. No. 553519 , C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553928, left panel or biotinylated mAb SL156 Cat. No. 551863, right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 , followed by Streptavidin-APC Cat. No. 554067 and FITC-conjugated anti-mouse CD45R/B220 mAb Cat. No. 553087/553088 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Two-color analysis of Pre-B cell receptor Pre-BCR expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM b mAb AF6-78 Cat. No. 553519 , C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553928, left panel or biotinylated mAb SL156 Cat. No. 551863, right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 , followed by Streptavidin-APC Cat. No. 554067 and FITC-conjugated anti-mouse CD45R/B220 mAb Cat. No. 553087/553088 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Two-color analysis of Pre-B cell receptor Pre-BCR expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM b mAb AF6-78 Cat. No. 553519 , C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553928, left panel or biotinylated mAb SL156 Cat. No. 551863, right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 , followed by Streptavidin-APC Cat. No. 554067 and FITC-conjugated anti-mouse CD45R/B220 mAb Cat. No. 553087/553088 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Two-color analysis of Pre-B cell receptor Pre-BCR expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM b mAb AF6-78 Cat. No. 553519 , C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, kappa isotype control mAb R35-95 Cat. No. 553928, left panel or biotinylated mAb SL156 Cat. No. 551863, right panel in the presence of Mouse BD Fc Block purified anti-mouse CD16/CD32 mAb 2.4G2 Cat. No. 553141/553142 , followed by Streptavidin-APC Cat. No. 554067 and FITC-conjugated anti-mouse CD45R/B220 mAb Cat. No. 553087/553088 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system.