BD PharmingenTM Calcein AM

Description

Flow cytometric analysis of BD Pharmingen Calcein AM fluorescence in Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 20 hours and then stained with 10 muM BD Pharmingen Calcein AM Cat. No. 564061; solid line histograms in serum-free buffer. Unstained cells are indicated by the dashed line histograms. Non-viable cells show a decrease in fluorescence intensity. Histograms were derived from gated events with the light scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Calcein AM has also been tested on mouse data not shown . Fluorescent staining of human U-2 OS cells with BD Pharmingen Calcein AM. Cells from the U-2 OS Osteosarcoma, ATCC HTB-96 cell line were stained with 10 muM BD Pharmingen Calcein AM pseudo-colored green in serum-free buffer. The cells were counterstained with DRAQ5 Cat. No. 564903/564902 pseudo-colored red . The images were captured using a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Flow cytometric analysis of BD Pharmingen Calcein AM fluorescence in Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 20 hours and then stained with 10 muM BD Pharmingen Calcein AM Cat. No. 564061; solid line histograms in serum-free buffer. Unstained cells are indicated by the dashed line histograms. Non-viable cells show a decrease in fluorescence intensity. Histograms were derived from gated events with the light scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Calcein AM has also been tested on mouse data not shown . Show More Fluorescent staining of human U-2 OS cells with BD Pharmingen Calcein AM. Cells from the U-2 OS Osteosarcoma, ATCC HTB-96 cell line were stained with 10 muM BD Pharmingen Calcein AM pseudo-colored green in serum-free buffer. The cells were counterstained with DRAQ5 Cat. No. 564903/564902 pseudo-colored red . The images were captured using a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Show More Flow cytometric analysis of BD Pharmingen Calcein AM fluorescence in Jurkat Cells. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 20 hours and then stained with 10 muM BD Pharmingen Calcein AM Cat. No. 564061; solid line histograms in serum-free buffer. Unstained cells are indicated by the dashed line histograms. Non-viable cells show a decrease in fluorescence intensity. Histograms were derived from gated events with the light scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Calcein AM has also been tested on mouse data not shown . Fluorescent staining of human U-2 OS cells with BD Pharmingen Calcein AM. Cells from the U-2 OS Osteosarcoma, ATCC HTB-96 cell line were stained with 10 muM BD Pharmingen Calcein AM pseudo-colored green in serum-free buffer. The cells were counterstained with DRAQ5 Cat. No. 564903/564902 pseudo-colored red . The images were captured using a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software.