
559934 · 200 Tests
BDB Bioscience · Cat: 559934
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Cy5 Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated top left & top right panels or treated for 6 hours bottom left & bottom right panels with 6 muM camptothecin. Cells were incubated with Cy5 Annexin V and analyzed by flow cytometry. Left panels are representative of an experiment analyzed on a BD FACSCalibur instrument and right panels from an experiment analyzed on a BD FACSVantage instrument. Untreated cells were primarily Cy5 Annexin V negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour treatment with camptothecin, there were two populations of cells: cells undergoing apoptosis Cy5 Annexin V positive , and cells that were viable and not undergoing apoptosis Cy5 Annexin V negative . Cy5 Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated top left & top right panels or treated for 6 hours bottom left & bottom right panels with 6 muM camptothecin. Cells were incubated with Cy5 Annexin V and analyzed by flow cytometry. Left panels are representative of an experiment analyzed on a BD FACSCalibur instrument and right panels from an experiment analyzed on a BD FACSVantage instrument. Untreated cells were primarily Cy5 Annexin V negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour treatment with camptothecin, there were two populations of cells: cells undergoing apoptosis Cy5 Annexin V positive , and cells that were viable and not undergoing apoptosis Cy5 Annexin V negative . Show More Cy5 Annexin V: A tool for identifying cells that are undergoing apoptosis. Jurkat T cells were left untreated top left & top right panels or treated for 6 hours bottom left & bottom right panels with 6 muM camptothecin. Cells were incubated with Cy5 Annexin V and analyzed by flow cytometry. Left panels are representative of an experiment analyzed on a BD FACSCalibur instrument and right panels from an experiment analyzed on a BD FACSVantage instrument. Untreated cells were primarily Cy5 Annexin V negative, indicating that they were viable and not undergoing apoptosis. After a 4 hour treatment with camptothecin, there were two populations of cells: cells undergoing apoptosis Cy5 Annexin V positive , and cells that were viable and not undergoing apoptosis Cy5 Annexin V negative .