BD PharmingenTM DRAQ7TM

Description

Panel 1. Two-color flow cytometric analysis of Jurkat cell viability. Jurkat cells were treated with 5 muM Camptothecin Right Plot or DMSO vehicle Left Plot overnight. Cells were resuspended in Annexin V Binding Buffer Cat. No. 556454 and stained with FITC Annexin V Cat. No. 556419 and 1.25 muM DRAQ7 Cat. No. 564904 . Camptothecin-treated cells show an increased frequency of apoptotic Annexin V+ DRAQ7- and dead Annexin V+ DRAQ7+ cells. Analysis was performed using a BD LSRFortessa Cell Analyzer System. Panel 2. Flow cytometric analysis of HeLa cell DNA content. Cultured HeLa cells in log phase growth were harvested using Gibco Cell Dissociation Buffer Life Technologies , fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Cells were resuspended in DPBS with 20 muM DRAQ7 and analyzed using a low BD LSRFortessa cytometer flow rate. Histograms were deconvoluted by FlowJo software into G0/G1, S, and G2/M populations. Panel 3. Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 44 were cultured on mTeSR1 medium StemCell Technologies and fixed with BD Cytofix fixation buffer Cat. No. 554655 . The fixed cells were stained with Alexa Fluor 488 Mouse anti-Human Alkaline Phosphatase monoclonal antibody pseudo-colored green, Cat. No. 561495 . And 5 muM DRAQ7 pseudo-colored red was used as a nuclear counterstain. The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. DRAQ7 was also tested in mouse data not shown . Panel 1. Two-color flow cytometric analysis of Jurkat cell viability. Jurkat cells were treated with 5 muM Camptothecin Right Plot or DMSO vehicle Left Plot overnight. Cells were resuspended in Annexin V Binding Buffer Cat. No. 556454 and stained with FITC Annexin V Cat. No. 556419 and 1.25 muM DRAQ7 Cat. No. 564904 . Camptothecin-treated cells show an increased frequency of apoptotic Annexin V+ DRAQ7- and dead Annexin V+ DRAQ7+ cells. Analysis was performed using a BD LSRFortessa Cell Analyzer System. Panel 2. Flow cytometric analysis of HeLa cell DNA content. Cultured HeLa cells in log phase growth were harvested using Gibco Cell Dissociation Buffer Life Technologies , fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Cells were resuspended in DPBS with 20 muM DRAQ7 and analyzed using a low BD LSRFortessa cytometer flow rate. Histograms were deconvoluted by FlowJo software into G0/G1, S, and G2/M populations. Panel 3. Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 44 were cultured on mTeSR1 medium StemCell Technologies and fixed with BD Cytofix fixation buffer Cat. No. 554655 . The fixed cells were stained with Alexa Fluor 488 Mouse anti-Human Alkaline Phosphatase monoclonal antibody pseudo-colored green, Cat. No. 561495 . And 5 muM DRAQ7 pseudo-colored red was used as a nuclear counterstain. The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. DRAQ7 was also tested in mouse data not shown . Show More Panel 1. Two-color flow cytometric analysis of Jurkat cell viability. Jurkat cells were treated with 5 muM Camptothecin Right Plot or DMSO vehicle Left Plot overnight. Cells were resuspended in Annexin V Binding Buffer Cat. No. 556454 and stained with FITC Annexin V Cat. No. 556419 and 1.25 muM DRAQ7 Cat. No. 564904 . Camptothecin-treated cells show an increased frequency of apoptotic Annexin V+ DRAQ7- and dead Annexin V+ DRAQ7+ cells. Analysis was performed using a BD LSRFortessa Cell Analyzer System. Panel 2. Flow cytometric analysis of HeLa cell DNA content. Cultured HeLa cells in log phase growth were harvested using Gibco Cell Dissociation Buffer Life Technologies , fixed, and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Cells were resuspended in DPBS with 20 muM DRAQ7 and analyzed using a low BD LSRFortessa cytometer flow rate. Histograms were deconvoluted by FlowJo software into G0/G1, S, and G2/M populations. Panel 3. Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 44 were cultured on mTeSR1 medium StemCell Technologies and fixed with BD Cytofix fixation buffer Cat. No. 554655 . The fixed cells were stained with Alexa Fluor 488 Mouse anti-Human Alkaline Phosphatase monoclonal antibody pseudo-colored green, Cat. No. 561495 . And 5 muM DRAQ7 pseudo-colored red was used as a nuclear counterstain. The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. DRAQ7 was also tested in mouse data not shown .