BD PharmingenTM FITC Annexin V

Description

FITC Annexin V: A tool for identifying cells that are undergoing apoptosis. HBP-ALL human leukemia cells were left untreated top left & bottom left panels , treated for 5 hr top middle & bottom middle panels or 12 hr top right & bottom right panels with anti-human Fas antibody Clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with FITC Annexin V in a buffer containing propidium iodide PI and analyzed by flow cytometry. Untreated cells were primarily FITC Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. After a 5 hr treatment with DX2, the majority of the cells were either undergoing apoptosis FITC Annexin V positive and PI negative or had already died FITC Annexin V and PI positive . After a 12 hr treatment with DX2, the majority of the cells had already died FITC Annexin V and PI positive . The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by cross-linking the Fas receptor. FITC Annexin V: A tool for identifying cells that are undergoing apoptosis. HBP-ALL human leukemia cells were left untreated top left & bottom left panels , treated for 5 hr top middle & bottom middle panels or 12 hr top right & bottom right panels with anti-human Fas antibody Clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with FITC Annexin V in a buffer containing propidium iodide PI and analyzed by flow cytometry. Untreated cells were primarily FITC Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. After a 5 hr treatment with DX2, the majority of the cells were either undergoing apoptosis FITC Annexin V positive and PI negative or had already died FITC Annexin V and PI positive . After a 12 hr treatment with DX2, the majority of the cells had already died FITC Annexin V and PI positive . The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by cross-linking the Fas receptor. Show More FITC Annexin V: A tool for identifying cells that are undergoing apoptosis. HBP-ALL human leukemia cells were left untreated top left & bottom left panels , treated for 5 hr top middle & bottom middle panels or 12 hr top right & bottom right panels with anti-human Fas antibody Clone DX2, Cat. No. 555670 and Protein G. Cells were incubated with FITC Annexin V in a buffer containing propidium iodide PI and analyzed by flow cytometry. Untreated cells were primarily FITC Annexin V and PI negative, indicating that they were viable and not undergoing apoptosis. After a 5 hr treatment with DX2, the majority of the cells were either undergoing apoptosis FITC Annexin V positive and PI negative or had already died FITC Annexin V and PI positive . After a 12 hr treatment with DX2, the majority of the cells had already died FITC Annexin V and PI positive . The addition of Protein G enhances the ability of DX2 to induce apoptosis, presumably by cross-linking the Fas receptor.