BD PharmingenTM FITC Mouse Anti-Human CD371 (Clec12A)

Description

Flow cytometric analysis of human CD371 Clec12A expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells PBMC were stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 556652 or FITC Mouse Anti-Human CD371 Clec12A Cat. No. 562569 antibody. The cells were also stained with PE-Cy7 Mouse Anti-Human HLA-DR Cat. No. 560651 , Alexa Fluor 700 Mouse Anti-Human CD11c Cat. No. 561352 , PE-Cy5 Mouse Anti-Human CD123 Cat. No. 551065 , BD Horizon PE-CF594 Mouse Anti-Human CD14 Cat. No. 562334 and a lineage cocktail comprised of BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 , CD14 Cat. No. 560350 , CD19 Cat. No. 560354 and CD56 Cat. No. 560361 . Histograms showing the expression of CD371 solid line or Ig isotype control staining dotted line on CD14+ monocytes Left Panel , Lineage-HLA-DR+CD11c+ myeloid dendritic cells Middle Panel and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells Right Panel were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes for dendritic cells , respectively. Flow cytometry was performed using a BD LSRII Flow Cytometer System. Flow cytometric analysis of human CD371 Clec12A expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells PBMC were stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 556652 or FITC Mouse Anti-Human CD371 Clec12A Cat. No. 562569 antibody. The cells were also stained with PE-Cy7 Mouse Anti-Human HLA-DR Cat. No. 560651 , Alexa Fluor 700 Mouse Anti-Human CD11c Cat. No. 561352 , PE-Cy5 Mouse Anti-Human CD123 Cat. No. 551065 , BD Horizon PE-CF594 Mouse Anti-Human CD14 Cat. No. 562334 and a lineage cocktail comprised of BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 , CD14 Cat. No. 560350 , CD19 Cat. No. 560354 and CD56 Cat. No. 560361 . Histograms showing the expression of CD371 solid line or Ig isotype control staining dotted line on CD14+ monocytes Left Panel , Lineage-HLA-DR+CD11c+ myeloid dendritic cells Middle Panel and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells Right Panel were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes for dendritic cells , respectively. Flow cytometry was performed using a BD LSRII Flow Cytometer System. Flow cytometric analysis of human CD371 Clec12A expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells PBMC were stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 556652 or FITC Mouse Anti-Human CD371 Clec12A Cat. No. 562569 antibody. The cells were also stained with PE-Cy7 Mouse Anti-Human HLA-DR Cat. No. 560651 , Alexa Fluor 700 Mouse Anti-Human CD11c Cat. No. 561352 , PE-Cy5 Mouse Anti-Human CD123 Cat. No. 551065 , BD Horizon PE-CF594 Mouse Anti-Human CD14 Cat. No. 562334 and a lineage cocktail comprised of BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 , CD14 Cat. No. 560350 , CD19 Cat. No. 560354 and CD56 Cat. No. 560361 . Histograms showing the expression of CD371 solid line or Ig isotype control staining dotted line on CD14+ monocytes Left Panel , Lineage-HLA-DR+CD11c+ myeloid dendritic cells Middle Panel and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells Right Panel were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes for dendritic cells , respectively. Flow cytometry was performed using a BD LSRII Flow Cytometer System. Show More Flow cytometric analysis of human CD371 Clec12A expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells PBMC were stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 556652 or FITC Mouse Anti-Human CD371 Clec12A Cat. No. 562569 antibody. The cells were also stained with PE-Cy7 Mouse Anti-Human HLA-DR Cat. No. 560651 , Alexa Fluor 700 Mouse Anti-Human CD11c Cat. No. 561352 , PE-Cy5 Mouse Anti-Human CD123 Cat. No. 551065 , BD Horizon PE-CF594 Mouse Anti-Human CD14 Cat. No. 562334 and a lineage cocktail comprised of BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 , CD14 Cat. No. 560350 , CD19 Cat. No. 560354 and CD56 Cat. No. 560361 . Histograms showing the expression of CD371 solid line or Ig isotype control staining dotted line on CD14+ monocytes Left Panel , Lineage-HLA-DR+CD11c+ myeloid dendritic cells Middle Panel and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells Right Panel were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes for dendritic cells , respectively. Flow cytometry was performed using a BD LSRII Flow Cytometer System. Show More Flow cytometric analysis of human CD371 Clec12A expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells PBMC were stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 556652 or FITC Mouse Anti-Human CD371 Clec12A Cat. No. 562569 antibody. The cells were also stained with PE-Cy7 Mouse Anti-Human HLA-DR Cat. No. 560651 , Alexa Fluor 700 Mouse Anti-Human CD11c Cat. No. 561352 , PE-Cy5 Mouse Anti-Human CD123 Cat. No. 551065 , BD Horizon PE-CF594 Mouse Anti-Human CD14 Cat. No. 562334 and a lineage cocktail comprised of BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 , CD14 Cat. No. 560350 , CD19 Cat. No. 560354 and CD56 Cat. No. 560361 . Histograms showing the expression of CD371 solid line or Ig isotype control staining dotted line on CD14+ monocytes Left Panel , Lineage-HLA-DR+CD11c+ myeloid dendritic cells Middle Panel and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells Right Panel were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes for dendritic cells , respectively. Flow cytometry was performed using a BD LSRII Flow Cytometer System. Flow cytometric analysis of human CD371 Clec12A expression on monocytes and dendritic cell subsets from human PBMC. Human peripheral blood mononuclear cells PBMC were stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 556652 or FITC Mouse Anti-Human CD371 Clec12A Cat. No. 562569 antibody. The cells were also stained with PE-Cy7 Mouse Anti-Human HLA-DR Cat. No. 560651 , Alexa Fluor 700 Mouse Anti-Human CD11c Cat. No. 561352 , PE-Cy5 Mouse Anti-Human CD123 Cat. No. 551065 , BD Horizon PE-CF594 Mouse Anti-Human CD14 Cat. No. 562334 and a lineage cocktail comprised of BD Horizon V450 Mouse Anti-Human CD3 Cat. No. 560366 , CD14 Cat. No. 560350 , CD19 Cat. No. 560354 and CD56 Cat. No. 560361 . Histograms showing the expression of CD371 solid line or Ig isotype control staining dotted line on CD14+ monocytes Left Panel , Lineage-HLA-DR+CD11c+ myeloid dendritic cells Middle Panel and Lineage-HLA-DR+CD123+ plasmacytoid dendritic cells Right Panel were derived from gated events with the forward and side light-scatter characteristics of viable monocytes or lymphocytes for dendritic cells , respectively. Flow cytometry was performed using a BD LSRII Flow Cytometer System.