BD PharmingenTM FITC Mouse anti-Human Granzyme B

Description

Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse Lysing buffer Cat. No. 555899 prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 APC- RPA-T8, Cat. No. 555369 and either mouse anti-human granzyme B antibody FITC- GB11, Cat. No. 558132 , filled histograms or immunoglobulin isotype control FITC- MOPC-21, Cat. No. 555909 , empty histograms by using Pharmingens staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody 5 mug, data not shown prior to staining. The histograms in the figure were derived from CD8-positive Left panel or CD8 -negative Right panel gated events. Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse Lysing buffer Cat. No. 555899 prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 APC- RPA-T8, Cat. No. 555369 and either mouse anti-human granzyme B antibody FITC- GB11, Cat. No. 558132 , filled histograms or immunoglobulin isotype control FITC- MOPC-21, Cat. No. 555909 , empty histograms by using Pharmingens staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody 5 mug, data not shown prior to staining. The histograms in the figure were derived from CD8-positive Left panel or CD8 -negative Right panel gated events. Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse Lysing buffer Cat. No. 555899 prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 APC- RPA-T8, Cat. No. 555369 and either mouse anti-human granzyme B antibody FITC- GB11, Cat. No. 558132 , filled histograms or immunoglobulin isotype control FITC- MOPC-21, Cat. No. 555909 , empty histograms by using Pharmingens staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody 5 mug, data not shown prior to staining. The histograms in the figure were derived from CD8-positive Left panel or CD8 -negative Right panel gated events. Show More Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse Lysing buffer Cat. No. 555899 prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 APC- RPA-T8, Cat. No. 555369 and either mouse anti-human granzyme B antibody FITC- GB11, Cat. No. 558132 , filled histograms or immunoglobulin isotype control FITC- MOPC-21, Cat. No. 555909 , empty histograms by using Pharmingens staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody 5 mug, data not shown prior to staining. The histograms in the figure were derived from CD8-positive Left panel or CD8 -negative Right panel gated events. Show More Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse Lysing buffer Cat. No. 555899 prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 APC- RPA-T8, Cat. No. 555369 and either mouse anti-human granzyme B antibody FITC- GB11, Cat. No. 558132 , filled histograms or immunoglobulin isotype control FITC- MOPC-21, Cat. No. 555909 , empty histograms by using Pharmingens staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody 5 mug, data not shown prior to staining. The histograms in the figure were derived from CD8-positive Left panel or CD8 -negative Right panel gated events. Expression of granzyme B by CD8 positive and CD8 negative peripheral blood mononuclear cells. Whole human blood was lysed with PharmLyse Lysing buffer Cat. No. 555899 prior to staining with GB11. The lysed human blood was subsequently fixed, permeablilized and stained with APC- conjugated mouse anti-human CD8 APC- RPA-T8, Cat. No. 555369 and either mouse anti-human granzyme B antibody FITC- GB11, Cat. No. 558132 , filled histograms or immunoglobulin isotype control FITC- MOPC-21, Cat. No. 555909 , empty histograms by using Pharmingens staining protocol. To demonstrate specificity of staining, the binding of FITC-GB11 was blocked by preincubation of the fixed/permeabilized cells with an excess of unlabelled GB11 antibody 5 mug, data not shown prior to staining. The histograms in the figure were derived from CD8-positive Left panel or CD8 -negative Right panel gated events.