BD PharmingenTM FITC Mouse Anti-Human IFN-gamma

Description

Expression of IFN-gamma by stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE Mouse Anti-Human CD3epsilon Cat. No. 556612 , fixed, permeabilized, and subsequently stained with FITC Mouse Anti-Human IFN-gamma Cat. No. 552882/554551/561057 . The two-color dot plot was derived from gated events with the side and forward light-scattering characteristics of viable cells. Expression of IFN-gamma by stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE Mouse Anti-Human CD3epsilon Cat. No. 556612 , fixed, permeabilized, and subsequently stained with FITC Mouse Anti-Human IFN-gamma Cat. No. 552882/554551/561057 . The two-color dot plot was derived from gated events with the side and forward light-scattering characteristics of viable cells. Show More Expression of IFN-gamma by stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . Rhesus PBMC were stimulated for 6 hr with PMA 50 ng/ml; Sigma and calcium ionophore A23187 500 ng/ml; Sigma in the presence of 2 muM BD GolgiStop Cat. No. 554724 . The PBMC were stained with PE Mouse Anti-Human CD3epsilon Cat. No. 556612 , fixed, permeabilized, and subsequently stained with FITC Mouse Anti-Human IFN-gamma Cat. No. 552882/554551/561057 . The two-color dot plot was derived from gated events with the side and forward light-scattering characteristics of viable cells.