BD PharmingenTM FITC Mouse Anti-Human MIP-1beta

Description

Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with BD GolgiStop Cat. No. 554715 , and stained with either a FITC Mouse IgG1, kappa isotype control Cat. No. 555909; shaded or with the FITC Mouse Anti-Human MIP-1beta antibody Cat. No. 560565; unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with GolgiStop , and stained with the FITC Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with BD GolgiStop Cat. No. 554715 , and stained with either a FITC Mouse IgG1, kappa isotype control Cat. No. 555909; shaded or with the FITC Mouse Anti-Human MIP-1beta antibody Cat. No. 560565; unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with GolgiStop , and stained with the FITC Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with BD GolgiStop Cat. No. 554715 , and stained with either a FITC Mouse IgG1, kappa isotype control Cat. No. 555909; shaded or with the FITC Mouse Anti-Human MIP-1beta antibody Cat. No. 560565; unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with GolgiStop , and stained with the FITC Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with BD GolgiStop Cat. No. 554715 , and stained with either a FITC Mouse IgG1, kappa isotype control Cat. No. 555909; shaded or with the FITC Mouse Anti-Human MIP-1beta antibody Cat. No. 560565; unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with GolgiStop , and stained with the FITC Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with BD GolgiStop Cat. No. 554715 , and stained with either a FITC Mouse IgG1, kappa isotype control Cat. No. 555909; shaded or with the FITC Mouse Anti-Human MIP-1beta antibody Cat. No. 560565; unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with GolgiStop , and stained with the FITC Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of MIP-1beta on human PBMC. Human PBMC were stimulated with 20 ng/mL human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Cat. No. 554724 . Left Panel: The PBMC were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with BD GolgiStop Cat. No. 554715 , and stained with either a FITC Mouse IgG1, kappa isotype control Cat. No. 555909; shaded or with the FITC Mouse Anti-Human MIP-1beta antibody Cat. No. 560565; unshaded . Right Panel: Both unstimulated shaded and stimulated PBMC unshaded were harvested, fixed, permeabilized with BD Cytofix/CytoPerm Plus Kit with GolgiStop , and stained with the FITC Mouse Anti-Human MIP-1beta antibody. Histograms were derived from gated events based on light scattering characteristics for monocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.