BD PharmingenTM FITC Mouse Anti-Human PD-L2 (CD273)

Description

Flow cytometric analysis of PD-L2 CD273 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 and then stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 553456; dashed line histogram or FITC Mouse Anti-Human PD-L2 CD273 antibody Cat. No. 569833/569908; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing PD-L2 CD273 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of PD-L2 CD273 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 and then stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 553456; dashed line histogram or FITC Mouse Anti-Human PD-L2 CD273 antibody Cat. No. 569833/569908; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing PD-L2 CD273 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of PD-L2 CD273 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were preincubated with Human BD Fc Block Cat. No. 564219/564220 and then stained with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 553456; dashed line histogram or FITC Mouse Anti-Human PD-L2 CD273 antibody Cat. No. 569833/569908; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing PD-L2 CD273 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.