BD PharmingenTM FITC Mouse Anti-Human TCR Cbeta1

Description

Two-color flow cytometric analysis of TCR Cbeta1 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426; Left Plots , APC Mouse Anti-Human CD4 Cat. No. 561841; Center Plots , or BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795; Right Plots antibodies and with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 553456; Upper Plots or FITC Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 571219/571217; Lower Plots at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of TCR Cbeta1 or Ig Isotype control staining versus CD3, CD4, or CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of TCR Cbeta1 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426; Left Plots , APC Mouse Anti-Human CD4 Cat. No. 561841; Center Plots , or BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795; Right Plots antibodies and with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 553456; Upper Plots or FITC Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 571219/571217; Lower Plots at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of TCR Cbeta1 or Ig Isotype control staining versus CD3, CD4, or CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of TCR Cbeta1 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon BV421 Mouse Anti-Human CD3 Cat. No. 562426; Left Plots , APC Mouse Anti-Human CD4 Cat. No. 561841; Center Plots , or BD Horizon BUV395 Mouse Anti-Human CD8 Cat. No. 563795; Right Plots antibodies and with either FITC Mouse IgG2a, kappa Isotype Control Cat. No. 553456; Upper Plots or FITC Mouse Anti-Human TCR Cbeta1 antibody Cat. No. 571219/571217; Lower Plots at 0.5 mug/test. The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of TCR Cbeta1 or Ig Isotype control staining versus CD3, CD4, or CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.