BD PharmingenTM FITC Mouse Anti-Human TNF

Description

Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334 , fixed, permeabilized, and subsequently stained with 0.25 mug of FITC Mouse Anti-Human TNF Cat. No. 554512/562082/552889, left panel . To demonstrate specificity of staining, the binding of FITC Mouse Anti-Human TNF was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF 5 mug, Cat. No. 554618; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510; right panel prior to staining with the FITC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334 , fixed, permeabilized, and subsequently stained with 0.25 mug of FITC Mouse Anti-Human TNF Cat. No. 554512/562082/552889, left panel . To demonstrate specificity of staining, the binding of FITC Mouse Anti-Human TNF was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF 5 mug, Cat. No. 554618; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510; right panel prior to staining with the FITC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334 , fixed, permeabilized, and subsequently stained with 0.25 mug of FITC Mouse Anti-Human TNF Cat. No. 554512/562082/552889, left panel . To demonstrate specificity of staining, the binding of FITC Mouse Anti-Human TNF was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF 5 mug, Cat. No. 554618; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510; right panel prior to staining with the FITC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334 , fixed, permeabilized, and subsequently stained with 0.25 mug of FITC Mouse Anti-Human TNF Cat. No. 554512/562082/552889, left panel . To demonstrate specificity of staining, the binding of FITC Mouse Anti-Human TNF was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF 5 mug, Cat. No. 554618; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510; right panel prior to staining with the FITC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls. Show More Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334 , fixed, permeabilized, and subsequently stained with 0.25 mug of FITC Mouse Anti-Human TNF Cat. No. 554512/562082/552889, left panel . To demonstrate specificity of staining, the binding of FITC Mouse Anti-Human TNF was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF 5 mug, Cat. No. 554618; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510; right panel prior to staining with the FITC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls. Expression of TNF by stimulated human peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of GolgiStop 2 muM final concentration; Cat. No. 554715 . The PBMC were stained with PE-Cy5 Mouse Anti-Human CD3 Cat. No. 555334 , fixed, permeabilized, and subsequently stained with 0.25 mug of FITC Mouse Anti-Human TNF Cat. No. 554512/562082/552889, left panel . To demonstrate specificity of staining, the binding of FITC Mouse Anti-Human TNF was blocked by the preincubation of the fluorochrome-conjugated antibody with recombinant human TNF 5 mug, Cat. No. 554618; middle panel , and by preincubation of the fixed/permeabilized cells with Purified Mouse Anti-Human TNF 5 mug, Cat. No. 554510; right panel prior to staining with the FITC Mouse Anti-Human TNF. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking and unlabeled antibody blocking specificity controls.