
559585 ·
BDB Bioscience · Cat: 559585
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CD4 expression on peripheral blood lymphocytes . Pig whole blood was stained with either FITC-conjugated 74-12-4 monoclonal antibody shaded histogram or FITC-conjugated Mouse IgG2b, kappa isotype control mAb MPC11 open histogram, Cat. no. 559532 . Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. no. 555816/555815 , and lymphocytes were identified by scatter profile. Flow cytometry was performed on a BD FACScan flow cytometry system. CD4 expression on peripheral blood lymphocytes . Pig whole blood was stained with either FITC-conjugated 74-12-4 monoclonal antibody shaded histogram or FITC-conjugated Mouse IgG2b, kappa isotype control mAb MPC11 open histogram, Cat. no. 559532 . Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. no. 555816/555815 , and lymphocytes were identified by scatter profile. Flow cytometry was performed on a BD FACScan flow cytometry system. Show More CD4 expression on peripheral blood lymphocytes . Pig whole blood was stained with either FITC-conjugated 74-12-4 monoclonal antibody shaded histogram or FITC-conjugated Mouse IgG2b, kappa isotype control mAb MPC11 open histogram, Cat. no. 559532 . Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. no. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. no. 555816/555815 , and lymphocytes were identified by scatter profile. Flow cytometry was performed on a BD FACScan flow cytometry system.