
551303 ·
BDB Bioscience · Cat: 551303
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained with either FITC-conjugated 76-2-11 mAb shaded histogram or FITC-conjugated mouse Igg2a, kappa isotype control mAb G155-178 Cat. No. 553456, open histogram . Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. No. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. No. 555816/555815 , and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained with either FITC-conjugated 76-2-11 mAb shaded histogram or FITC-conjugated mouse Igg2a, kappa isotype control mAb G155-178 Cat. No. 553456, open histogram . Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. No. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. No. 555816/555815 , and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More CD8 expression on peripheral blood lymphocytes. Pig whole blood was stained with either FITC-conjugated 76-2-11 mAb shaded histogram or FITC-conjugated mouse Igg2a, kappa isotype control mAb G155-178 Cat. No. 553456, open histogram . Erythrocytes were lysed BD Pharm Lyse lysis buffer, Cat. No. 555899 , non-viable leukocytes were excluded by staining with 7-AAD BD Via-Probe cell viability dye, Cat. No. 555816/555815 , and lymphocytes were gated according to scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.