BD PharmingenTM FITC Mouse IgG2b, kappa Isotype Control

Description

Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 1.0 mug/ml final concentration; Sigma, Cat. No.L-8274 in the presence of BD GolgiStop 2 muM final concentration; Cat. No. 554724 . PBMCs were then harvested, stained with 0.25 mug of PE Mouse Anti-Human CD14 Cat. No. 555398 , fixed, permeabilized, and subsequently stained with either 0.125 mug of FITC-anti-human IL-8 left panel , or 0.125 mug of FITC Mouse IgG2b, kappa Isotype Control Cat. No. 555057; middle panel . The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 1.0 mug/ml final concentration; Sigma, Cat. No.L-8274 in the presence of BD GolgiStop 2 muM final concentration; Cat. No. 554724 . PBMCs were then harvested, stained with 0.25 mug of PE Mouse Anti-Human CD14 Cat. No. 555398 , fixed, permeabilized, and subsequently stained with either 0.125 mug of FITC-anti-human IL-8 left panel , or 0.125 mug of FITC Mouse IgG2b, kappa Isotype Control Cat. No. 555057; middle panel . The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 1.0 mug/ml final concentration; Sigma, Cat. No.L-8274 in the presence of BD GolgiStop 2 muM final concentration; Cat. No. 554724 . PBMCs were then harvested, stained with 0.25 mug of PE Mouse Anti-Human CD14 Cat. No. 555398 , fixed, permeabilized, and subsequently stained with either 0.125 mug of FITC-anti-human IL-8 left panel , or 0.125 mug of FITC Mouse IgG2b, kappa Isotype Control Cat. No. 555057; middle panel . The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Show More Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 1.0 mug/ml final concentration; Sigma, Cat. No.L-8274 in the presence of BD GolgiStop 2 muM final concentration; Cat. No. 554724 . PBMCs were then harvested, stained with 0.25 mug of PE Mouse Anti-Human CD14 Cat. No. 555398 , fixed, permeabilized, and subsequently stained with either 0.125 mug of FITC-anti-human IL-8 left panel , or 0.125 mug of FITC Mouse IgG2b, kappa Isotype Control Cat. No. 555057; middle panel . The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Show More Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 1.0 mug/ml final concentration; Sigma, Cat. No.L-8274 in the presence of BD GolgiStop 2 muM final concentration; Cat. No. 554724 . PBMCs were then harvested, stained with 0.25 mug of PE Mouse Anti-Human CD14 Cat. No. 555398 , fixed, permeabilized, and subsequently stained with either 0.125 mug of FITC-anti-human IL-8 left panel , or 0.125 mug of FITC Mouse IgG2b, kappa Isotype Control Cat. No. 555057; middle panel . The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel . Expression of human IL-8 by stimulated CD14+ human monocytes. Human PBMCs were stimulated for 6 hours with LPS 1.0 mug/ml final concentration; Sigma, Cat. No.L-8274 in the presence of BD GolgiStop 2 muM final concentration; Cat. No. 554724 . PBMCs were then harvested, stained with 0.25 mug of PE Mouse Anti-Human CD14 Cat. No. 555398 , fixed, permeabilized, and subsequently stained with either 0.125 mug of FITC-anti-human IL-8 left panel , or 0.125 mug of FITC Mouse IgG2b, kappa Isotype Control Cat. No. 555057; middle panel . The data reflect gating on monocytes, based on forward and side scattered light signals. The quadrant markers for the bivariate dot plot were set based on autofluorescence controls right panel .