BD PharmingenTM FITC Rat Anti-Human IL-2

Description

Flow cytometric analysis of IL-2 expression on stimulated Rhesus macaque Macaca mulatta peripheral blood mononuclear cells. Rhesus PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of 2 muM GolgiStop Cat. No. 554724 . The PBMCs were stained with PE Mouse Anti-Human CD3 Cat. No. 555333/561808/561809 , fixed, permeabilized, and then stained with FITC Rat Anti-Human IL-2 0.25 mug; Cat. No. 554565/561055/559361 . The quadrant markers for the bivariate dot plot was set based on the autofluorescence control, and verified with the blocking controls. Flow cytometric analysis of IL-2 expression on stimulated Rhesus macaque Macaca mulatta peripheral blood mononuclear cells. Rhesus PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of 2 muM GolgiStop Cat. No. 554724 . The PBMCs were stained with PE Mouse Anti-Human CD3 Cat. No. 555333/561808/561809 , fixed, permeabilized, and then stained with FITC Rat Anti-Human IL-2 0.25 mug; Cat. No. 554565/561055/559361 . The quadrant markers for the bivariate dot plot was set based on the autofluorescence control, and verified with the blocking controls. Show More Flow cytometric analysis of IL-2 expression on stimulated Rhesus macaque Macaca mulatta peripheral blood mononuclear cells. Rhesus PBMC were stimulated for 6 hours with PMA Sigma, Cat. #P-8139 and calcium ionophore A23187 Sigma, Cat. #C-9275 in the presence of 2 muM GolgiStop Cat. No. 554724 . The PBMCs were stained with PE Mouse Anti-Human CD3 Cat. No. 555333/561808/561809 , fixed, permeabilized, and then stained with FITC Rat Anti-Human IL-2 0.25 mug; Cat. No. 554565/561055/559361 . The quadrant markers for the bivariate dot plot was set based on the autofluorescence control, and verified with the blocking controls.