BD PharmingenTM FITC Rat Anti-Mouse CD200R3

Description

Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either FITC Rat IgG2a, kappa Isotype Control Cat. No. 554688; Left Plot or FITC Rat Anti-Mouse CD200R3 antibody Cat. No. 567837; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either FITC Rat IgG2a, kappa Isotype Control Cat. No. 554688; Left Plot or FITC Rat Anti-Mouse CD200R3 antibody Cat. No. 567837; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either FITC Rat IgG2a, kappa Isotype Control Cat. No. 554688; Left Plot or FITC Rat Anti-Mouse CD200R3 antibody Cat. No. 567837; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either FITC Rat IgG2a, kappa Isotype Control Cat. No. 554688; Left Plot or FITC Rat Anti-Mouse CD200R3 antibody Cat. No. 567837; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either FITC Rat IgG2a, kappa Isotype Control Cat. No. 554688; Left Plot or FITC Rat Anti-Mouse CD200R3 antibody Cat. No. 567837; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either FITC Rat IgG2a, kappa Isotype Control Cat. No. 554688; Left Plot or FITC Rat Anti-Mouse CD200R3 antibody Cat. No. 567837; Right Plot at 1 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.