BD PharmingenTM FITC Rat Anti-Mouse CD73

Description

Multicolor flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/C Bottom Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565/565992 and either FITC Rat IgG1, kappa Isotype Control Cat. No. 553924; Left Plots or FITC Rat Anti-Mouse CD73 antibody Cat. No. 567701; Right Plots at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/C Bottom Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565/565992 and either FITC Rat IgG1, kappa Isotype Control Cat. No. 553924; Left Plots or FITC Rat Anti-Mouse CD73 antibody Cat. No. 567701; Right Plots at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD73 expression on splenic leucocytes from two mouse strains. C57BL/6 Top Plots and BALB/C Bottom Plots mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565/565992 and either FITC Rat IgG1, kappa Isotype Control Cat. No. 553924; Left Plots or FITC Rat Anti-Mouse CD73 antibody Cat. No. 567701; Right Plots at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD73 or Ig Isotype control staining versus CD3e were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.